Daniel P, Desmet G
Biomed Pharmacother. 1986;40(9):338-41.
A rapid technique for measuring cellular density in microplates cultures is described. Cells are stained by toluidine blue (0% to 1% acetic acid), washed in 1% acetic acid and digested by trypsin (1%) or pronase (0.1%). Absorbance rate is read in an automated spectrophotometer for microplates. The correlation coefficient between absorbance and cellular number is higher than 0.99. The measures accuracy is satisfactory in the experimental conditions used.
描述了一种用于测量微孔板培养物中细胞密度的快速技术。细胞用甲苯胺蓝（0%至1%乙酸）染色，在1%乙酸中洗涤，并用胰蛋白酶（1%）或链霉蛋白酶（0.1%）消化。在微孔板自动分光光度计中读取吸光度率。吸光度与细胞数量之间的相关系数高于0.99。在所使用的实验条件下，测量准确性令人满意。
