Laboratório de Cultivo de Tejidos Vegetales, Colegio de Postgraduados Campus Cordoba, Amatlan de los Reyes, Veracruz, Mexico.
Universidad Autónoma Metropolitana, Unidad Iztapalapa, Vicentina, Iztapalapa, Mexico.
Methods Mol Biol. 2024;2759:227-244. doi: 10.1007/978-1-0716-3654-1_21.
Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air. The current review resumes the application of TIS in orchid propagation. Fifty-three papers are discussed considering: explant, culture media, TIS bioreactor type, frequency and immersion time, and the TIS effects in acclimatization phase.
临时浸没系统（TIS）已被用于兰花的微繁殖。TIS 用于微繁殖的主要优点是外植体定期浸没在营养培养基中，然后排干营养溶液，使外植体组织留在空气中。本综述总结了 TIS 在兰花繁殖中的应用。讨论了 53 篇论文，考虑了外植体、培养基、TIS 生物反应器类型、频率和浸没时间以及 TIS 在适应阶段的影响。
