West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
Methods Mol Biol. 2024;2753:283-306. doi: 10.1007/978-1-0716-3625-1_14.
Exogenous teratogens contribute to approximately 10% of the human abnormality with exposure occurrence during the prenatal and fetal period. However, the assessment methods and underlying mechanism remain unclear. The nematode Caenorhabditis elegans has been recognized as one of the ideal model animals for toxicologic research as convenient culture, low cost, and complete phenotypes and genomic profiling. This chapter describes the protocols about the estimations on the teratogenic effects using nematodes as model organisms, including the growth, development, behavior, reproduction, energy balance, and transgenes.
外源性致畸剂约占人类异常的 10%，其暴露发生在产前和胎儿期。然而，其评估方法和潜在机制尚不清楚。秀丽隐杆线虫已被认为是毒理学研究的理想模式动物之一，因为其培养方便、成本低、表型和基因组图谱完整。本章介绍了使用线虫作为模型生物评估致畸作用的方案，包括生长、发育、行为、繁殖、能量平衡和转基因。
