State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Methods Mol Biol. 2024;2771:111-118. doi: 10.1007/978-1-0716-3702-9_15.
Mycoviruses exist in all major groups of fungi. With the continuous development of science and technology, the methods of studying viruses are constantly updated, and progressively mycoviruses have been discovered where most of these viruses are RNA viruses. Therefore, double-stranded RNA has traditionally been used as the hallmark of RNA mycovirus detection. This report describes in detail the method of mycovirus identification using extraction of dsRNA. Besides, extraction of viral dsRNA, and the assembly methods of viral genome and identification of virus type are presented.
真菌病毒广泛存在于各类真菌中。随着科学技术的不断发展，病毒的研究方法也在不断更新，越来越多的真菌病毒被发现，其中大部分是 RNA 病毒。因此，双链 RNA 一直以来都被用作 RNA 真菌病毒检测的标志。本报告详细描述了利用 dsRNA 提取进行真菌病毒鉴定的方法。此外，还介绍了病毒 dsRNA 的提取、病毒基因组的组装方法以及病毒类型的鉴定。
