Stäubli M, Roessler B, Straub P W
Blut. 1987 Apr;54(4):239-45. doi: 10.1007/BF00594200.
125I albumin was used to assess the amount of trapped fluid after microhematocrit centrifugation of erythrocytes suspended in buffers of different osmolality. Surprisingly the total amount of trapped fluid per volume unit of packed erythrocytes decreased with decreasing osmolality of the suspending buffer despite erythrocyte swelling. However, if the contribution of the individual erythrocyte to the trapped fluid was calculated, the trapped fluid per erythrocyte did not change between 311 mosm/kg and 256 mosm/kg. For osmolalities below 256 mosm/kg a significant increase of trapped fluid was obtained. It is concluded that the packing ability of erythrocytes is not impaired in suspending fluid of moderate to severe infraphysiological tonicity. The daily clinical experience that considerable degrees of plasma hypoosmolality are tolerated in vivo without hemolysis or impairment of oxygen transport by erythrocytes may be explained by the excellent ability of shape adaptation of erythrocytes to each other and to other surfaces such as vascular endothelia. The method of trapped fluid determination might be of potential value as a complementary method in the evaluation of erythrocyte rheology if the amount of trapped fluid is related to the individual erythrocyte.
用¹²⁵I白蛋白评估悬浮于不同渗透压缓冲液中的红细胞经微量血细胞比容离心后截留的液体量。令人惊讶的是，尽管红细胞肿胀，但每单位体积压实红细胞截留的液体总量随着悬浮缓冲液渗透压的降低而减少。然而，如果计算单个红细胞对截留液体的贡献，在311毫渗摩尔/千克和256毫渗摩尔/千克之间每个红细胞截留的液体量没有变化。对于低于256毫渗摩尔/千克的渗透压，截留的液体量显著增加。结论是，在中度至重度低于生理张力的悬浮液中，红细胞的聚集能力未受损。在体内，相当程度的血浆低渗可被耐受而不发生溶血或红细胞氧运输受损，这一日常临床经验可能是由于红细胞彼此之间以及与血管内皮等其他表面具有出色的形状适应能力。如果截留的液体量与单个红细胞相关，截留液体量测定方法作为评估红细胞流变学的一种补充方法可能具有潜在价值。
