Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão, 277, 05508-090, SP, Brazil.
Instituto de Pesquisas Ambientais, Avenida Miguel Estéfano, 3687, 04301-012, SP, Brazil.
Mol Omics. 2024 Mar 25;20(3):203-212. doi: 10.1039/d3mo00209h.
Plants should be probably thought of as the most formidable chemical laboratory that can be exploited for the production of an incredible number of molecules with remarkable structural and chemical diversity that cannot be matched by any synthetic libraries of small molecules. The bryophytes chemistry has been neglected for too long, but in the last ten years, this scenery is changing, with several studies being made using extracts from bryophytes, aimed at the characterization of interesting metabolites, with their metabolome screened. The main objective of this study was to analyze the metabolome of , a native Brazilian moss species, which occurs in the two Brazilian hotspots. GC-MS and LC-MS were performed. All extracts were analyzed using the molecular networking approach. The four extracts of (polar, non-polar, soluble, and insoluble) resulted in 928 features detected within the established parameters. 189 (20.4%) compounds were annotated, with sugars, fatty acids, flavonoids, and biflavonoids as the major constituents. Sucrose was the sugar with the highest quantity; palmitic acid the major fatty acid but with great presence of very long-chain fatty acids rarely found in higher plants, glycosylated flavonoids were the major flavonoids, and biflavonoids majorly composed by units of flavones and flavanones, exclusively found in the cell wall. Despite the high percentage, this work leaves a significant gap for future works using other structure elucidation techniques, such as NMR.
植物应该被认为是最强大的化学实验室，可以用来生产数量惊人的具有显著结构和化学多样性的分子，这些分子是任何小分子合成库都无法比拟的。苔藓植物化学长期以来一直被忽视，但在过去的十年中，这种情况正在发生变化，有几项研究使用苔藓植物提取物进行，旨在对有趣的代谢物进行表征，并对其代谢组进行筛选。本研究的主要目的是分析巴西本土苔藓物种的代谢组，该物种存在于巴西的两个热点地区。进行了 GC-MS 和 LC-MS 分析。使用分子网络方法分析所有提取物。四种 的提取物（极性、非极性、可溶和不溶）在设定的参数内检测到 928 个特征。鉴定出 189 种（20.4%）化合物，主要成分有糖、脂肪酸、类黄酮和双黄酮。蔗糖是含量最高的糖；棕榈酸是主要的脂肪酸，但存在大量在高等植物中很少见的超长链脂肪酸，糖基化类黄酮是主要的类黄酮，双黄酮主要由黄酮和黄烷酮单元组成，仅存在于细胞壁中。尽管这一比例很高，但这项工作为未来使用其他结构解析技术（如 NMR）的工作留下了很大的空白。
