Lichtenstein A
Cancer Immunol Immunother. 1987;24(2):172-7. doi: 10.1007/BF00205596.
The antitumor effects of the streptococcal preparation OK-432 were analyzed in a murine ovarian teratocarcinoma (MOT) model. Administration of OK-432 i.p. prevented tumor outgrowth in 75% of mice challenged with 10(3) MOT cells i.p. 24 h previously. Treatment was less successful in mice challenged with 10(4) or 10(5) cells, preventing tumor growth in 25% of the former and only 5% of the latter group. Tumor-challenged mice cured by injections of OK-432 were not rendered resistant to a subsequent challenge with 10(3) MOT cells 75 days after initial treatment. Only the i.p. route of administration was effective as i.v. OK-432 did not prolong survival of tumor-challenged mice. An antitumor response was detected as early as 24 h after i.p. treatment. This correlated temporally with an influx of neutrophils into the peritoneal cavity. Peritoneal cells obtained between 6 and 24 h after treatment were capable of lysing MOT targets in vitro. A single cell cytotoxicity assay demonstrated that peritoneal neutrophils, elicited by i.p. injection of OK-432, could bind to and lyse MOT targets. These data indicate that OK-432 is effective against small tumor cell inocula in this murine model of ovarian cancer and, furthermore, that the neutrophilic response into the peritoneal cavity plays a role in tumor rejection.
在小鼠卵巢畸胎瘤（MOT）模型中分析了链球菌制剂OK-432的抗肿瘤作用。腹腔注射OK-432可使75%在24小时前腹腔接种10³个MOT细胞的小鼠肿瘤不生长。对于接种10⁴或10⁵个细胞的小鼠，治疗效果较差，分别只有25%和5%的小鼠肿瘤生长受到抑制。经OK-432注射治愈的荷瘤小鼠在初始治疗75天后，对随后10³个MOT细胞的攻击没有产生抗性。只有腹腔注射途径有效，静脉注射OK-432不能延长荷瘤小鼠的生存期。腹腔注射治疗后24小时即可检测到抗肿瘤反应。这在时间上与中性粒细胞流入腹腔相关。治疗后6至24小时获得的腹腔细胞能够在体外裂解MOT靶细胞。单细胞细胞毒性试验表明，腹腔注射OK-432诱导的腹腔中性粒细胞能够结合并裂解MOT靶细胞。这些数据表明，在该小鼠卵巢癌模型中，OK-432对小剂量肿瘤细胞接种有效，此外，腹腔内的中性粒细胞反应在肿瘤排斥中起作用。
