Bourret E, Bardet L
Clin Chim Acta. 1980 Mar 14;102(1):1-9. doi: 10.1016/0009-8981(80)90427-1.
The experimental conditions for single radial haemolysis are analysed in order to optimize the haemolytic plate method for the assay of human total complement. The amount of haemolytic antibody used for sensitization of red cells, the concentration of sensitized sheep erythrocytes, the thickness of agarose layer, and the diameters of wells all exercise an influence on the area of haemolysis. The effects of these parameters are investigated with a view to obtaining the clearest reading and greatest sensitivity of the haemolytic activity plate method, and at the same time verifying the validity of the assay and the precision of the human serum haemolytic titres. This work reports the optimal experimental requirements with regard to the factors studied for minimizing the sources of error in complement haemolytic plate assay.
分析单径向溶血的实验条件，以优化用于检测人总补体的溶血平板法。用于红细胞致敏的溶血抗体量、致敏绵羊红细胞的浓度、琼脂糖层的厚度以及孔的直径均对溶血面积有影响。研究这些参数的作用，旨在获得溶血活性平板法最清晰的读数和最高的灵敏度，同时验证该检测方法的有效性以及人血清溶血滴度的精密度。本文报道了关于所研究因素的最佳实验要求，以尽量减少补体溶血平板检测中的误差来源。
