Samuditha Pathirannahalage Sahan, Adassooriya Nadeesh Madusanka, Salim Nazeera
Department of Botany, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
Department of Chemical and Process Engineering, Faculty of Engineering, University of Peradeniya, Peradeniya, Sri Lanka.
Beilstein J Nanotechnol. 2024 Jan 23;15:115-125. doi: 10.3762/bjnano.15.11. eCollection 2024.
The escalating release of zinc oxide nanoparticles (ZnO NPs) into the environment poses a substantial threat, potentially leading to increased concentrations of zinc (Zn) in the soil and subsequent phytotoxic effects. This study aimed to assess the effects of ZnO NPs on () concerning its tolerance levels, toxicity, and accumulation. ZnO NPs were synthesized by the wet chemical method and characterized by powder X-ray diffraction (PXRD), Fourier-transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM). The effect of ZnO NPs (70 nm) on grown in coir was evaluated. The application of 1,000 mg/L of ZnO NPs resulted in a significant increase ( < 0.05) in soluble protein content, carbohydrates, chlorophyll a (Chl-a), chlorophyll b (Chl-b), total chlorophylls, carotenoids, and antioxidants by 24.7%, 58.5%, 38.0%, 42.2%, 39.9%, 11.2%, and 7.7%, respectively. Interestingly, this dose had no impact on the indole acetic acid (IAA) content. Conversely, the use of 2,000 mg/L of ZnO NPs in the same medium led to a significant reduction ( < 0.05) in soluble protein content by 23.1%, accompanied by a notable increase in IAA by 31.1%, indicating potential toxicity. The use of atomic absorption spectroscopy confirmed the internalization of zinc in seedlings, with a statistically significant increase ( < 0.05). In control plants without ZnO NPs, Zn concentration was 0.36 mg/g, while at the highest ZnO NPs tested dose of 10,000 mg/L, it significantly rose to 1.76 mg/g, causing leaf chlorosis and stunted seedling growth. This suggests potential health risks related to Zn toxicity for consumers. Given the adverse effects on at concentrations above 1000 mg/L, caution is advised in the application and release of ZnO NPs, highlighting the importance of responsible practices to mitigate harm to plant life and consumer health. The study demonstrated the tolerance of to high Zn levels, classifying it as a Zn-tolerant species.
氧化锌纳米颗粒（ZnO NPs）向环境中的释放不断增加，构成了重大威胁，可能导致土壤中锌（Zn）浓度升高，进而产生植物毒性效应。本研究旨在评估ZnO NPs对（）在耐受性水平、毒性和积累方面的影响。通过湿化学方法合成了ZnO NPs，并通过粉末X射线衍射（PXRD）、傅里叶变换红外（FTIR）光谱、紫外可见（UV-vis）光谱、动态光散射（DLS）和扫描电子显微镜（SEM）对其进行了表征。评估了70纳米的ZnO NPs对椰壳中生长的（）的影响。施加1000毫克/升的ZnO NPs导致可溶性蛋白质含量、碳水化合物、叶绿素a（Chl-a）、叶绿素b（Chl-b）、总叶绿素、类胡萝卜素和抗氧化剂显著增加（P<0.05），分别增加了24.7%、58.5%、38.0%、42.2%、39.9%、11.2%和7.7%。有趣的是，该剂量对吲哚乙酸（IAA）含量没有影响。相反，在相同培养基中使用2000毫克/升的ZnO NPs导致可溶性蛋白质含量显著降低（P<0.05），降低了23.1%，同时IAA显著增加了31.1%，表明存在潜在毒性。使用原子吸收光谱法证实了锌在幼苗中的内化，具有统计学显著增加（P<0.05）。在没有ZnO NPs的对照植物中，锌浓度为0.36毫克/克，而在测试的最高ZnO NPs剂量10000毫克/升时，显著升至至1.76毫克/克，导致叶片黄化和幼苗生长发育迟缓。这表明与锌毒性相关的潜在健康风险对消费者存在影响。鉴于浓度高于1000毫克/升时对（）有不利影响，建议在ZnO NPs的应用和释放方面谨慎行事，突出了采取负责任措施减轻对植物生命和消费者健康危害的重要性。该研究证明了（）对高锌水平的耐受性，将其归类为耐锌物种。
