Abdelaziz Amer M, Salem Salem S, Khalil Ahmed M A, El-Wakil Deiaa A, Fouda Hossam M, Hashem Amr H
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, 11884, Cairo, Egypt.
Biology Department, College of Science, Taibah University, Yanbu, 41911, Kingdom of Saudi Arabia.
Biometals. 2022 Jun;35(3):601-616. doi: 10.1007/s10534-022-00391-8. Epub 2022 Mar 31.
In this study, a novel, non-toxic, eco-friendly zinc oxide nanoparticles (ZnO-NPs) was used instead of the synthetic fungicides widely used to control the destructive phytopathogenic fungus Fusarium oxysporum, the causative agent of wilt disease in Solanum melongena L. Herein, the biosynthesized ZnO-NPs was carried out by Penicillium expansum ATCC 7861. In vitro, mycosynthesized ZnO-NPs exhibited antifungal activity against Fusarium oxysporum. In vivo, ZnO-NPs suppressed Fusarium wilt disease in cultivated Solanum melongena L. by decreasing the disease severity with 75% of plant protection. Moreover, ZnO-NPs stimulated the recovery of eggplant as an indicated by improving of morphological and metabolic indicators including plant height(152.5%), root length(106.6%), plant fresh biomass (146%), chlorophyll a (102.8%), chlorophyll b (67.86%), total soluble carbohydrates (48.5%), total soluble protein (81.8%), phenol (10.5%), antioxidant activity and isozymes compared with infected control. Therefore, this study suggests using mycosynthesized ZnO-NPs as an alternative to synthetic fungicides not only to eradicate the Fusarium wilt disease in cultivated eggplant (Solanum melongena) but also to promote the growth parameters and metabolic aspects.
在本研究中，使用了一种新型、无毒、环保的氧化锌纳米颗粒（ZnO-NPs）来替代广泛用于防治毁灭性植物病原真菌尖孢镰刀菌（Fusarium oxysporum）的合成杀菌剂，尖孢镰刀菌是茄子（Solanum melongena L.）枯萎病的病原体。在此，由扩展青霉（Penicillium expansum）ATCC 7861进行生物合成ZnO-NPs。在体外，真菌合成的ZnO-NPs对尖孢镰刀菌表现出抗真菌活性。在体内，ZnO-NPs通过将病害严重程度降低75%的植物保护作用，抑制了栽培茄子中的枯萎病。此外，ZnO-NPs促进了茄子的恢复，这表现为形态和代谢指标的改善，包括株高（152.5%）、根长（106.6%）、植物鲜生物量（146%）、叶绿素a（102.8%）、叶绿素b（67.86%）、总可溶性碳水化合物（48.5%）、总可溶性蛋白（81.8%）、酚类（10.5%）、抗氧化活性和同工酶，与感染对照相比。因此，本研究表明，使用真菌合成的ZnO-NPs作为合成杀菌剂的替代品，不仅可以根除栽培茄子（Solanum melongena）中的枯萎病，还可以促进生长参数和代谢方面的改善。
