Department of Pharmaceutics, China Pharmaceutical University, Nanjing, 211100, People's Republic of China.
Children's Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Int J Nanomedicine. 2024 Jan 25;19:845-858. doi: 10.2147/IJN.S436391. eCollection 2024.
Rapid detection and diagnosis of diseases facilitate timely and effective treatment of cardiovascular diseases (CVD). The establishment of a one-step rapid detection method provides a new method for the initial screening and disease risk assessment of patients with cardiovascular diseases in primary medical units.
Hollow gold nanoparticles (HGNPs) were synthesized using a cobalt template method followed by use as signal amplification probes for ultra-sensitive quantitative detection of serum C-reactive protein (CRP). To induce the localized surface plasmon resonance (LSPR) and improve protein labeling efficiency, we developed a sensitive detection mode by coating polyvinylpyrrolidone (PVP-K30) on the HGNPs, resulting in a significant improvement in detection performance.
Compared to traditional colloidal GNP-based LFTA, PVP-coated HGNPs exhibit a lower visual detection limit of 1 ng/mL, which a 25-fold decrement compare to using GNPs as the antibody-labeled probe, and the detection limit could be reduced to 0.14 ng/mL under the quantitative instrument.
The one-step method based on HGNP immunochromatographic strips modified with PVP established in this study can be used for the detection of CRP and hs-CRP in biological samples. The performance of the immunochromatographic technique designed in this study was evaluated from the perspective of synthetic markers, and the application conditions of this strip were screened, verifying its high specificity, indicating that it has high sensitivity and strong detection limit compared to colloidal gold. The sensitivity of the hollow gold immunochromatographic test strip in this article has been increased by about 25 times, providing a new method for rapid detection of CVD in clinical diagnosis.
快速检测和诊断疾病有助于及时有效地治疗心血管疾病（CVD）。建立一步快速检测方法为基层医疗机构心血管疾病患者的初步筛查和疾病风险评估提供了新方法。
采用钴模板法合成空心金纳米粒子（HGNPs），作为超灵敏定量检测血清 C 反应蛋白（CRP）的信号放大探针。为了诱导局域表面等离子体共振（LSPR）并提高蛋白质标记效率，我们通过在 HGNPs 上涂覆聚乙烯吡咯烷酮（PVP-K30）开发了一种敏感的检测模式，从而显著提高了检测性能。
与传统基于胶体 GNP 的 LFTA 相比，PVP 包覆的 HGNPs 的目视检测限低至 1ng/mL，比使用 GNP 作为抗体标记探针降低了 25 倍，在定量仪器下检测限可降低至 0.14ng/mL。
本研究建立的基于 PVP 修饰的 HGNP 免疫层析条的一步法可用于生物样品中 CRP 和 hs-CRP 的检测。从合成标志物的角度评价了免疫层析技术的性能，并筛选了该条带的应用条件，验证了其高特异性，表明与胶体金相比，该条带具有高灵敏度和较强的检测限。本文中空心金免疫层析试验条的灵敏度提高了约 25 倍，为临床诊断中 CVD 的快速检测提供了一种新方法。
