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SHR 和 SCR 协调细胞周期早期的根模式和生长。

SHR and SCR coordinate root patterning and growth early in the cell cycle.

机构信息

Department of Biology, Duke University, Durham, NC, USA.

Howard Hughes Medical Institute, Duke University, Durham, NC, USA.

出版信息

Nature. 2024 Feb;626(7999):611-616. doi: 10.1038/s41586-023-06971-z. Epub 2024 Jan 31.

DOI:10.1038/s41586-023-06971-z
PMID:38297119
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10866714/
Abstract

Precise control of cell division is essential for proper patterning and growth during the development of multicellular organisms. Coordination of formative divisions that generate new tissue patterns with proliferative divisions that promote growth is poorly understood. SHORTROOT (SHR) and SCARECROW (SCR) are transcription factors that are required for formative divisions in the stem cell niche of Arabidopsis roots. Here we show that levels of SHR and SCR early in the cell cycle determine the orientation of the division plane, resulting in either formative or proliferative cell division. We used 4D quantitative, long-term and frequent (every 15 min for up to 48 h) light sheet and confocal microscopy to probe the dynamics of SHR and SCR in tandem within single cells of living roots. Directly controlling their dynamics with an SHR induction system enabled us to challenge an existing bistable model of the SHR-SCR gene-regulatory network and to identify key features that are essential for rescue of formative divisions in shr mutants. SHR and SCR kinetics do not align with the expected behaviour of a bistable system, and only low transient levels, present early in the cell cycle, are required for formative divisions. These results reveal an uncharacterized mechanism by which developmental regulators directly coordinate patterning and growth.

摘要

精确的细胞分裂控制对于多细胞生物发育过程中的正确模式形成和生长至关重要。对于形成新组织模式的成形分裂与促进生长的增殖分裂的协调，我们知之甚少。SHORTROOT（SHR）和 SCARECROW（SCR）是拟南芥根干细胞龛中成形分裂所必需的转录因子。在这里，我们表明细胞周期早期的 SHR 和 SCR 水平决定了分裂平面的方向，导致成形分裂或增殖分裂。我们使用 4D 定量、长期和频繁（长达 48 小时内每 15 分钟一次）的光片和共聚焦显微镜，在活体根的单个细胞中同时探测 SHR 和 SCR 的动力学。通过 SHR 诱导系统直接控制其动力学，使我们能够挑战 SHR-SCR 基因调控网络的现有双稳态模型，并确定对于挽救 shr 突变体中的成形分裂所必需的关键特征。SHR 和 SCR 的动力学与双稳态系统的预期行为不一致，并且仅在细胞周期早期存在低瞬时水平，对于成形分裂是必需的。这些结果揭示了一种未被表征的机制，通过该机制，发育调节剂可以直接协调模式形成和生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/1fe65f4e8f36/41586_2023_6971_Fig12_ESM.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/39ff6d650452/41586_2023_6971_Fig8_ESM.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/20df6faaf564/41586_2023_6971_Fig11_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/1fe65f4e8f36/41586_2023_6971_Fig12_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/403eabbd5c1e/41586_2023_6971_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/e685d04249ef/41586_2023_6971_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/eb3de04c3304/41586_2023_6971_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/4e09094bb834/41586_2023_6971_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/85af3268e3a4/41586_2023_6971_Fig5_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/a82a762c159b/41586_2023_6971_Fig6_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/f3259b5827f8/41586_2023_6971_Fig7_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/39ff6d650452/41586_2023_6971_Fig8_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/a1dcf5877abd/41586_2023_6971_Fig9_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/4324eccd5f93/41586_2023_6971_Fig10_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/20df6faaf564/41586_2023_6971_Fig11_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c246/10866714/1fe65f4e8f36/41586_2023_6971_Fig12_ESM.jpg

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