Liu Yunhao, Du Zhuolin, Li Ying, Lu Shaoping, Tang Shan, Guo Liang
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070 China.
Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan, 430070 China.
Mol Breed. 2024 Jan 29;44(2):9. doi: 10.1007/s11032-024-01445-0. eCollection 2024 Feb.
With the increasing public attention to the health benefit of polyunsaturated fatty acids (PUFAs) and demand for linolenic acid (C18:3), it is of great significance to increase the C18:3 content in our meal. As an oil crop with high content of C18:3, has three homologous copies of and three homologous copies . In this study, we seed-specifically overexpressed two fatty acid desaturase genes, and , in rapeseed cultivar Zhongshuang 9. The results show that C18:3 content in and overexpressed seeds is increased from 8.62% in wild-type (WT) to 10.62-12.95% and 14.54-26.16%, respectively. We crossed and overexpression lines, and stable homozygous digenic crossed lines were obtained. The C18:3 content was increased from 8.62% in WT to 28.46-53.57% in crossed overexpression lines. In addition, we found that the overexpression of and had no effect on rapeseed growth, development, and other agronomic traits. In conclusion, we successfully generated rapeseed germplasms with high C18:3 content by simultaneously overexpressing and , which provides a feasible way for breeding high C18:3 rapeseed cultivars.
The online version contains supplementary material available at 10.1007/s11032-024-01445-0.
随着公众对多不饱和脂肪酸（PUFAs）健康益处的关注度不断提高以及对亚麻酸（C18:3）需求的增加，提高我们膳食中C18:3的含量具有重要意义。作为一种C18:3含量高的油料作物，[作物名称]有三个[基因名称1]的同源拷贝和三个[基因名称2]的同源拷贝。在本研究中，我们在油菜品种中双9中种子特异性过表达了两个[脂肪酸去饱和酶基因名称]脂肪酸去饱和酶基因，[基因名称1]和[基因名称2]。结果表明，[基因名称1]和[基因名称2]过表达种子中的C18:3含量分别从野生型（WT）的8.62%增加到10.62 - 12.95%和14.54 - 26.16%。我们将[基因名称1]和[基因名称2]过表达系进行杂交，获得了稳定的纯合双基因杂交系。杂交过表达系中的C18:3含量从WT的8.62%增加到28.46 - 53.57%。此外，我们发现[基因名称1]和[基因名称2]的过表达对油菜的生长、发育和其他农艺性状没有影响。总之，我们通过同时过表达[基因名称1]和[基因名称2]成功培育出了C18:3含量高油菜种质，这为培育高C18:3油菜品种提供了一条可行的途径。
在线版本包含可在10.1007/s11032 - 024 - 01445 - 0获取的补充材料。
