State Key Laboratory of Digital Medical Engineering, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Life Science Research Center, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453100, China.
Small. 2024 Jul;20(27):e2310300. doi: 10.1002/smll.202310300. Epub 2024 Feb 1.
Glutathione (GSH) is the primary antioxidant in cells, and GSH consumption will break the redox balance in cells. Based on this, a method that uses high concentrations of GSH in the tumor microenvironment to trigger the redox reaction of Cu(II) to generate copper nanoprobes with fluorescence and tumor growth inhibition properties is proposed. The nanoprobe mainly exists in the form of Cu(I) and catalyzes the decomposition of hydrogen peroxide into hydroxyl radicals. At the same time, a simple and controllable carbon micro-nano electrode is used to construct a single-cell sensing platform, which enable the detection of glutathione content in single living cells after Cu(II) treatment, providing an excellent example for detecting single-cell biomolecules.
谷胱甘肽（GSH）是细胞内主要的抗氧化剂，GSH 的消耗会打破细胞内的氧化还原平衡。基于此，提出了一种利用肿瘤微环境中高浓度 GSH 触发 Cu(II)的氧化还原反应生成具有荧光和肿瘤生长抑制特性的铜纳米探针的方法。该纳米探针主要以 Cu(I)的形式存在，并催化过氧化氢分解为羟基自由基。同时，使用简单可控的碳微纳电极构建了单细胞传感平台，可在 Cu(II)处理后检测单个活细胞中的谷胱甘肽含量，为检测单细胞生物分子提供了一个极好的范例。
