Al-Asadi M J, Greenwood D, O'Grady F
Antimicrob Agents Chemother. 1979 Jul;16(1):77-80. doi: 10.1128/AAC.16.1.77.
A new model, which is designed to investigate the in vitro activity of antibiotics as a function of different concentration-time curves, is described. The antibiotic is allowed to diffuse through a membrane into a bacterial culture until a peak level is reached; the antibiotic is then removed by flow-assisted back diffusion. With this arrangement it is possible to expose bacteria to a changing concentration of drug while maintaining a constant volume of bacterial culture. Preliminary studies were carried out to investigate the response of a strain of Escherichia coli to gentamicin. The results indicate that bacteria surviving exposure to concentrations of gentamicin similar to those obtained during therapy may exhibit an increase in resistance to the antibiotic.
描述了一种新模型，该模型旨在研究抗生素的体外活性与不同浓度-时间曲线的关系。使抗生素通过膜扩散到细菌培养物中，直至达到峰值水平；然后通过流动辅助反向扩散去除抗生素。通过这种设置，可以使细菌暴露于不断变化的药物浓度中，同时保持细菌培养物的体积恒定。进行了初步研究以调查一株大肠杆菌对庆大霉素的反应。结果表明，在暴露于与治疗期间获得的浓度相似的庆大霉素浓度后仍存活的细菌可能会表现出对该抗生素的耐药性增加。
