Li Jingmei, Liang Jiadi, Chen Shanshan, Guo Wucheng, Chen Ting, Liu Xiqiang
Department of Stomatology, Nanfang Hospital, Southern Medical University Guangzhou 510515 P.R. China
Center of Stomatology, Shunde Hospital of Southern Medical University Foshan 528000 P.R. China.
RSC Adv. 2024 Feb 1;14(7):4416-4423. doi: 10.1039/d3ra08867g. eCollection 2024 Jan 31.
Although adhesive hydrogels represent an alternative to surgical sutures for non-invasive tissue wound sealing, those with indiscriminate adhesion fail to hold wounds while inhibiting postoperative tissue adhesion, thus limiting their application in intestinal repair. In this study, an asymmetric adhesive hydrogel sheet composed mainly of polyacrylic acid (PAA) and gelatin (GA) that can be wet-adhered to the surface of intestinal tissue was developed. One side of the GA-PAA hydrogel sheet was complexed with polyvinyl alcohol (PVA), which shielded the excess adhesion based on a physical barrier. Both sides of the PVA/GA-PAA hydrogel showed distinct adhesive and antiadhesive properties. Intriguingly, the anti-adhesive side showed significant anti-adhesion toward specific proteins. The results of animal experiments showed that the PVA/GA-PAA hydrogel could firmly adhere to the intestine to stop leakage and prevent post-operative tissue adhesion two weeks after surgery. The hematoxylin and eosin (H&E) staining results showed that the damaged intestinal serosa was repaired without tissue adhesion. It is believed that the controllable adhesion of the adhesive hydrogel offers better prospects for intestinal repair.
尽管粘性水凝胶是用于无创组织伤口封闭的手术缝合线的一种替代物，但那些具有非特异性粘附性的水凝胶在抑制术后组织粘连的同时无法闭合伤口，因此限制了它们在肠道修复中的应用。在本研究中，开发了一种主要由聚丙烯酸（PAA）和明胶（GA）组成的不对称粘性水凝胶片，其可湿粘附于肠道组织表面。GA-PAA水凝胶片的一侧与聚乙烯醇（PVA）复合，基于物理屏障屏蔽了过量的粘附作用。PVA/GA-PAA水凝胶的两侧表现出不同的粘附和抗粘附特性。有趣的是，抗粘附侧对特定蛋白质表现出显著的抗粘附作用。动物实验结果表明，PVA/GA-PAA水凝胶可牢固粘附于肠道以阻止渗漏，并在术后两周防止组织粘连。苏木精和伊红（H&E）染色结果表明，受损的肠浆膜得以修复且无组织粘连。据信，粘性水凝胶的可控粘附为肠道修复提供了更好的前景。
