Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Guangzhou, Guangdong 510070, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Guangzhou, Guangdong 510070, China.
Int J Food Microbiol. 2024 Mar 2;413:110603. doi: 10.1016/j.ijfoodmicro.2024.110603. Epub 2024 Jan 30.
Human noroviruses (HuNoVs) are the most predominant viral agents of acute gastroenteritis. Vegetables are important vehicles of HuNoVs transmission. This study aimed to assess the HuNoVs prevalence in vegetables. We searched the Web of Science, Excerpta Medica Database, PubMed, and Cochrane databases until June 1, 2023. A total of 27 studies were included for the meta-analysis. Statistical analysis was conducted using Stata 14.0 software. This analysis showed that the pooled HuNoVs prevalence in vegetables was 7 % (95 % confidence interval (CI): 3-13) worldwide. The continent with largest number of studies was Europe, and the highest number of samples was lettuce. As revealed by the results of the subgroup meta-analysis, the prevalence of GI genogroup was the highest (3 %, 95 % CI: 1-7). A higher prevalence was seen in vegetables from farms (18 %, 95 % CI: 5-37), while only 4 % (95 % CI: 1-8) in retail. The HuNoVs prevalence of ready-to-eat vegetables and non-ready-to-eat vegetables was 2 % (95 % CI: 0-8) and 9 % (95 % CI: 3-16), respectively. The prevalence by quantitative real time RT-PCR was 8 % (95 % CI: 3-15) compared to 3 % (95 % CI: 0-13) by conventional RT-PCR. Furthermore, the HuNoVs prevalence in vegetables was 6 % (95 % CI: 1-14) in ISO pretreatment method and 8 % (95 % CI: 1-19) in non-ISO method, respectively. This study is helpful in comprehensively understanding the prevalence of HuNoVs contamination in vegetables worldwide.
人类诺如病毒（HuNoVs）是急性胃肠炎的主要病毒性病原体。蔬菜是 HuNoVs 传播的重要载体。本研究旨在评估蔬菜中 HuNoVs 的流行情况。我们检索了 Web of Science、Excerpta Medica Database、PubMed 和 Cochrane 数据库，截至 2023 年 6 月 1 日。共有 27 项研究纳入荟萃分析。使用 Stata 14.0 软件进行统计分析。分析结果显示，全球蔬菜中 HuNoVs 的总流行率为 7%（95%置信区间：3-13）。研究数量最多的是欧洲，样本数量最多的是生菜。亚组荟萃分析结果显示，GI 基因群的流行率最高（3%，95%置信区间：1-7）。农场蔬菜的流行率较高（18%，95%置信区间：5-37），而零售蔬菜的流行率仅为 4%（95%置信区间：1-8）。即食蔬菜和非即食蔬菜的 HuNoVs 流行率分别为 2%（95%置信区间：0-8）和 9%（95%置信区间：3-16）。实时定量 RT-PCR 的流行率为 8%（95%置信区间：3-15），而常规 RT-PCR 的流行率为 3%（95%置信区间：0-13）。此外，ISO 预处理方法中蔬菜的 HuNoVs 流行率为 6%（95%置信区间：1-14），非 ISO 方法中为 8%（95%置信区间：1-19）。本研究有助于全面了解全球蔬菜中 HuNoVs 污染的流行情况。
