Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha, China.
Physiol Rep. 2024 Feb;12(3):e15929. doi: 10.14814/phy2.15929.
Diastolic dysfunction is a major cardiac dysfunction, and an important predisposing factor is age. Although exercise training is often used for the prevention and treatment of cardiovascular disease nowadays, little is currently known about whether exercise interventions associated with the slowing of cardiac aging are related to mtp-related pathways. In the present study, the UAS/Tub-Gal4 system was used to knockdown whole-body mtp expression levels in Drosophila, which underwent 2 weeks of endurance training. By conducting different assays and quantifying different indicators, we sought to investigate the relationship between mtp, exercise, and age-related diastolic dysfunction. We found that (1) Drosophila in the mtp youth group exhibited age-related diastolic dysfunction and had a significantly shorter mean lifespan. (2) Endurance exercise could improve diastolic dysfunction and prolong lifespan in aged Drosophila. (3) Endurance exercise could increase the expression levels of apolpp and Acox3, and decrease the levels of TC, LDL-C, and TG in the aged group. In summary, aging causes age-associated diastolic dysfunction in Drosophila, and systemic knockdown of mtp causes premature age-associated diastolic dysfunction in young Drosophila. Besides, endurance exercise improves age-related diastolic dysfunction and prolongs lifespan.
舒张功能障碍是一种主要的心脏功能障碍，一个重要的诱发因素是年龄。尽管运动训练目前常用于心血管疾病的预防和治疗，但目前还不清楚与减缓心脏衰老相关的运动干预是否与 mtp 相关途径有关。在本研究中，使用 UAS/Tub-Gal4 系统敲低果蝇全身 mtp 的表达水平，使其进行 2 周的耐力训练。通过进行不同的测定和量化不同的指标，我们试图研究 mtp、运动和与年龄相关的舒张功能障碍之间的关系。我们发现：（1）mtp 年轻组的果蝇表现出与年龄相关的舒张功能障碍，平均寿命明显缩短。（2）耐力运动可以改善老年果蝇的舒张功能障碍并延长其寿命。（3）耐力运动可以增加 apoLPP 和 Acox3 的表达水平，并降低老年组的 TC、LDL-C 和 TG 水平。综上所述，衰老导致果蝇出现与年龄相关的舒张功能障碍，全身敲低 mtp 会导致年轻果蝇出现过早的与年龄相关的舒张功能障碍。此外，耐力运动可以改善与年龄相关的舒张功能障碍并延长寿命。
