Kato Akinori
Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, 3327-204 Nakamachi, Nara, Nara 631-8505, Japan.
J Genomics. 2024 Jan 20;12:26-34. doi: 10.7150/jgen.91337. eCollection 2024.
is a species of Gram-negative bacteria that is found in soil, water, and sewage. is generally considered to be an opportunistic pathogen, but has also been reported as a plant growth-promoting bacterium (PGPB), which may have beneficial effects on plant growth and development. However, genetic analysis of has been limited, possibly due to its redundant enzymes that digest exogenous DNA in the cell. Here, an strain i6 was isolated from soil in Nara, Japan. This strain was amenable to transformation and the one-step gene inactivation method based on λ Red recombinase. The transformation efficiency of the i6 strain with the 10 kb plasmid DNA pCF430 was at least four orders of magnitude higher than that of the previously sequenced strain ATCC 35953, which could not be transformed with the same plasmid DNA. A draft genome sequence of the i6 strain was determined and deposited into the database, allowing several factors that may determine transformation efficiency to be perturbed and tested. Together with the amenability of the i6 strain to genetic manipulation, the information from the i6 genome will facilitate characterization and fine-tuning of the beneficial and detrimental traits of this species.
是一种革兰氏阴性菌，存在于土壤、水和污水中。通常被认为是一种机会致病菌，但也有报道称它是一种促进植物生长的细菌（PGPB），可能对植物生长和发育有有益影响。然而，对其的基因分析一直有限，可能是由于其细胞内存在消化外源DNA的冗余酶。在这里，从日本奈良的土壤中分离出了一株菌株i6。该菌株适合基于λ Red重组酶的转化和一步基因失活方法。i6菌株对10 kb质粒DNA pCF430的转化效率比先前测序的菌株ATCC 35953至少高四个数量级，而后者不能用相同的质粒DNA进行转化。测定了i6菌株的基因组草图序列并存入数据库，使得几个可能决定转化效率的因素能够被干扰和测试。结合i6菌株对基因操作的适应性，来自i6基因组的信息将有助于对该物种的有益和有害特性进行表征和微调。
