Liu Junlin, Zhou Zhangzhe, Hou Mingzhuang, Xia Xiaowei, Liu Yang, Zhao Zhijian, Wu Yubin, Deng Yaoge, Zhang Yijian, He Fan, Xu Yong, Zhu Xuesong
Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.
Orthopaedic Institute, Medical College, Soochow University, Suzhou 215007, China.
Mater Today Bio. 2024 Jan 24;25:100956. doi: 10.1016/j.mtbio.2024.100956. eCollection 2024 Apr.
The rational design of multifunctional biomaterials with hierarchical porous structure and on-demand biological activity is of great consequence for bone tissue engineering (BTE) in the contemporary world. The advanced combination of trace element cerium ions (Ce) with bone repair materials makes the composite material capable of promoting angiogenesis and enhancing osteoblast activity. Herein, a living and phosphorylated injectable porous hydrogel microsphere (P-GelMA-Ce@BMSCs) is constructed by microfluidic technology and coordination reaction with metal ion ligands while loaded with exogenous BMSCs. Exogenous stem cells can adhere to and proliferate on hydrogel microspheres, thus promoting cell-extracellular matrix (ECM) and cell-cell interactions. The active ingredient Ce promotes the proliferation, osteogenic differentiation of rat BMSCs, and angiogenesis of endotheliocytes by promoting mineral deposition, osteogenic gene expression, and VEGF secretion. The enhancement of osteogenesis and improvement of angiogenesis of the P-GelMA-Ce scaffold is mainly associated with the activation of the Wnt/β-catenin pathway. This study could provide novel and meaningful insights for treating bone defects with biofunctional materials on the basis of metal ions.
在当代，合理设计具有分级多孔结构和按需生物活性的多功能生物材料对骨组织工程（BTE）至关重要。微量元素铈离子（Ce）与骨修复材料的先进结合使复合材料能够促进血管生成并增强成骨细胞活性。在此，通过微流控技术和与金属离子配体的配位反应构建了一种负载外源性骨髓间充质干细胞（BMSCs）的活性且磷酸化的可注射多孔水凝胶微球（P-GelMA-Ce@BMSCs）。外源性干细胞可在水凝胶微球上黏附并增殖，从而促进细胞-细胞外基质（ECM）和细胞-细胞相互作用。活性成分Ce通过促进矿物质沉积、成骨基因表达和血管内皮生长因子（VEGF）分泌，促进大鼠骨髓间充质干细胞的增殖、成骨分化以及内皮细胞的血管生成。P-GelMA-Ce支架的成骨增强和血管生成改善主要与Wnt/β-连环蛋白通路的激活有关。本研究可为基于金属离子的生物功能材料治疗骨缺损提供新颖且有意义的见解。
