Wang Zheng-Hui, Peng Jing-Hui, Liu Yin-Cheng, Zhu Quan-Hua, Sun Chang, Xie Hui, Wang Shui
Department of Breast Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Women & Children Central Laboratory and Laboratory of Breast Disease Department, Jiangsu Women and Children Health Hospital, Nanjing, China.
Gland Surg. 2024 Jan 29;13(1):45-58. doi: 10.21037/gs-23-493. Epub 2024 Jan 19.
Adipose-derived stem cells (ADSCs) are closely associated with the survival rate of transplanted fat in breast reconstruction after breast cancer surgery. Nevertheless, the intrinsic mechanisms regulating ADSCs adipogenic differentiation remain ambiguous. The aim of our study was to explore the relevant genes and pathways to elucidate the potential mechanisms of adipogenic differentiation in ADSCs.
The Gene Expression Omnibus (GEO) dataset GSE61302 was downloaded and analyzed to identify differentially expressed genes (DEGs). Key genes and signaling pathways were obtained through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional and enrichment analysis. Protein-protein interaction (PPI) network and hub gene analyses were performed with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database and Cytoscape software. Finally, the transcription levels of hub genes in the adipogenic differentiated group and undifferentiated group of ADSCs were compared via real-time quantitative polymerase chain reaction (RT-qPCR).
In total, 1,091 DEGs were identified through bioinformatics analysis of the adipogenic differentiated group and undifferentiated group. If was then found that the 10 downregulated key genes, , and may play important roles in the adipogenic differentiation of ADSCs. Subsequent experimental verification also revealed that the messenger RNA (mRNA) expression levels of cyclin B1 in adipogenic differentiated cells and undifferentiated cells were significantly different at the early stage (P<0.05), but there was no significant difference at the late stage (P>0.05).
As a key gene, might be a potential biomarker in the adipogenic differentiation of ADSCs at the early stage.
脂肪来源干细胞（ADSCs）与乳腺癌手术后乳房重建中移植脂肪的存活率密切相关。然而，调节ADSCs成脂分化的内在机制仍不明确。本研究旨在探索相关基因和信号通路，以阐明ADSCs成脂分化的潜在机制。
下载并分析基因表达综合数据库（GEO）数据集GSE61302，以鉴定差异表达基因（DEGs）。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）功能及富集分析获得关键基因和信号通路。使用搜索互作基因/蛋白的工具（STRING）数据库和Cytoscape软件进行蛋白质-蛋白质相互作用（PPI）网络和枢纽基因分析。最后，通过实时定量聚合酶链反应（RT-qPCR）比较ADSCs成脂分化组和未分化组中枢纽基因的转录水平。
通过对成脂分化组和未分化组的生物信息学分析，共鉴定出1091个DEGs。随后发现10个下调的关键基因， ，以及 可能在ADSCs的成脂分化中起重要作用。后续实验验证还显示，成脂分化细胞和未分化细胞中细胞周期蛋白B1的信使核糖核酸（mRNA）表达水平在早期有显著差异（P<0.05），但在后期无显著差异（P>0.05）。
作为关键基因， 可能是ADSCs早期成脂分化的潜在生物标志物。
