Ferguson C A, Murray R G, Lancy P
Can J Microbiol. 1979 May;25(5):545-59. doi: 10.1139/m79-080.
A comparison was made of the effects of cis-tetrachlorodiaminoplatinum (IV) (cis-TCDPt), rans-TCDPt), and hexachloroplatinum (HCP) on growth and cell division of Escherichia coli strains D21 and D22. At or below 40 microgram/mL, cis-TCDPt inhibited cell division but not growth, DNA, or protein synthesis, although areas of increased electron density could be demonstrated in treated cells. In contrast, 40 microgram/mL of trans-TCDPt or HCP inhibited growth. Trans-TCDPt-treated cells developed condensed nucleoids; HCP-treated cells showed no obvious cytological changes to correlate with growth inhibition. Combination of cis-TCDPt with nalidixic acid, both at one-half the lowest filament-forming concentrations, resulted in formation of filaments, suggesting an additive effect. Combination of cis-TCDPt followed by ampicillin on E. coli B/r resulted in single bulges near the center of the filaments. Cis-TCDPt could therefore inhibit an initial step in the septation sequence, possibly at the level of the regulation of the hydrolytic enzymes. Whether cis-TCDPt exerts its effect by interreaction with DNA or with a membrane target is still uncertain.
对顺式四氯二氨合铂（IV）（顺式-TCDPt）、反式-TCDPt和六氯铂（HCP）对大肠杆菌菌株D21和D22生长及细胞分裂的影响进行了比较。在40微克/毫升及以下时，顺式-TCDPt抑制细胞分裂，但不抑制生长、DNA或蛋白质合成，尽管在处理过的细胞中可显示出电子密度增加的区域。相比之下，40微克/毫升的反式-TCDPt或HCP抑制生长。经反式-TCDPt处理的细胞形成浓缩的类核；经HCP处理的细胞未显示出与生长抑制相关的明显细胞学变化。顺式-TCDPt与萘啶酸以各自最低形成丝状体浓度的一半组合，导致丝状体形成，提示有相加作用。顺式-TCDPt后接氨苄西林对大肠杆菌B/r的组合导致在丝状体中心附近出现单个凸起。因此，顺式-TCDPt可能抑制隔膜形成序列中的初始步骤，可能是在水解酶调节水平。顺式-TCDPt是通过与DNA还是与膜靶点相互作用发挥其作用仍不确定。
