Delsarte A, Faway M, Frère J M, Coyette J, Calberg-Bacq C M, Heinen E
Antimicrob Agents Chemother. 1981 Mar;19(3):477-86. doi: 10.1128/AAC.19.3.477.
This paper presents a study of the interactions between Escherichia coli cells and nifurzide, a nitrofuran derivative which is used as an intestinal antiinfectious agent. At low concentrations of nifurzide, the growth rate of the cultures decreased, and elongated, nonseptate cells appeared. At high concentrations, complete growth inhibition occurred, accompanied by a rather strong bactericidal effect, but the appearance of the cells was normal; in particular, no bacteriolytic effect was observed. A very large number of antibiotic molecules were bound per bacterial cell. After cell disruption, similar amounts of nifurzide were found in the cytoplasm, cytoplasmic membranes, and cell wall, respectively. Most of the bound nifurzide was rapidly degraded or became protein bound. The structure of the outer membrane lipopolysaccharide appeared to have little influence on the activity of nifurzide.
本文介绍了一项关于大肠杆菌细胞与硝呋齐特（一种用作肠道抗感染剂的硝基呋喃衍生物）之间相互作用的研究。在低浓度的硝呋齐特下，培养物的生长速率下降，出现了细长的、无隔膜的细胞。在高浓度下，生长完全受到抑制，同时伴有相当强的杀菌作用，但细胞外观正常；特别是未观察到溶菌作用。每个细菌细胞结合了大量的抗生素分子。细胞破碎后，在细胞质、细胞质膜和细胞壁中分别发现了相似量的硝呋齐特。大多数结合的硝呋齐特迅速降解或与蛋白质结合。外膜脂多糖的结构似乎对硝呋齐特的活性影响很小。
