Burdett I D, Murray R G
J Bacteriol. 1974 Sep;119(3):1039-56. doi: 10.1128/jb.119.3.1039-1056.1974.
The formation of cell wall septa was monitored in Escherichia coli B and B/r during synchronous growth in glucose media at 37 C by means of electron microscopy. The visible events of septation comprised the following sequence, starting at about 30 min of incubation: (a) bleb formation of the outer membrane; (b) invagination of mucopeptide and cytoplasmic membrane (with associated mesosomes); the outer membrane is excluded from the septum; (c) formation of a cross-wall; (d) ingrowth of the outer membrane during cell separation. The septum is composed of a fold of cytoplasmic membrane plus mucopeptide, and the latter is a double structure, composed of two opposed lamellae separated by an electron-transparent gap. Experiments with chloramphenicol and nalidixic acid suggested that division could occur in the presence of these inhibitors once a round of deoxyribonucleic acid replication is completed. The initial stages of septation, as estimated by the potential of the cells to produce bulges in the presence of ampicillin, may involve the modification of mucopeptide by hydrolases at the end of the C period. Assembly of the septum may occur during the first half of the D period by means of precursors synthesized during the preceding C period.
通过电子显微镜监测了大肠杆菌B和B/r在37℃的葡萄糖培养基中同步生长期间细胞壁隔膜的形成。隔膜形成的可见事件包括以下顺序，从培养约30分钟开始：(a) 外膜形成泡；(b) 粘肽和细胞质膜（伴有间体）内陷；外膜被排除在隔膜之外；(c) 形成横壁；(d) 细胞分离期间外膜向内生长。隔膜由细胞质膜折叠加上粘肽组成，后者是一种双重结构，由两个相对的薄片组成，中间有一个电子透明间隙。氯霉素和萘啶酸实验表明，一旦一轮脱氧核糖核酸复制完成，在这些抑制剂存在的情况下仍可发生分裂。根据细胞在氨苄青霉素存在下产生凸起的能力估计，隔膜形成的初始阶段可能涉及C期末水解酶对粘肽的修饰。隔膜的组装可能在D期的前半段通过前一个C期合成的前体进行。
