Miroshnichenko Svetlana, Timofeeva Valeriia, Permykova Elizaveta, Ershov Sergey, Kiryukhantsev-Korneev Philip, Dvořaková Eva, Shtansky Dmitry V, Zajíčková Lenka, Solovieva Anastasiya, Manakhov Anton
Scientific Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russia.
Institute of Biochemistry ⁻ subdivision of the FRC FTM, 2 Timakova str., 630117 Novosibirsk, Russia.
Nanomaterials (Basel). 2019 Apr 19;9(4):637. doi: 10.3390/nano9040637.
Biodegradable nanofibers are extensively employed in different areas of biology and medicine, particularly in tissue engineering. The electrospun polycaprolactone (PCL) nanofibers are attracting growing interest due to their good mechanical properties and a low-cost structure similar to the extracellular matrix. However, the unmodified PCL nanofibers exhibit an inert surface, hindering cell adhesion and negatively affecting their further fate. The employment of PCL nanofibrous scaffolds for wound healing requires a certain modification of the PCL surface. In this work, the morphology of PCL nanofibers is optimized by the careful tuning of electrospinning parameters. It is shown that the modification of the PCL nanofibers with the COOH plasma polymers and the subsequent binding of NH groups of protein molecules is a rather simple and technologically accessible procedure allowing the adhesion, early spreading, and growth of human fibroblasts to be boosted. The behavior of fibroblasts on the modified PCL surface was found to be very different when compared to the previously studied cultivation of mesenchymal stem cells on the PCL nanofibrous meshes. It is demonstrated by X-ray photoelectron spectroscopy (XPS) that the freeze-thawed platelet-rich plasma (PRP) immobilization can be performed via covalent and non-covalent bonding and that it does not affect biological activity. The covalently bound components of PRP considerably reduce the fibroblast apoptosis and increase the cell proliferation in comparison to the unmodified PCL nanofibers or the PCL nanofibers with non-covalent bonding of PRP. The reported research findings reveal the potential of PCL matrices for application in tissue engineering, while the plasma modification with COOH groups and their subsequent covalent binding with proteins expand this potential even further. The use of such matrices with covalently immobilized PRP for wound healing leads to prolonged biological activity of the immobilized molecules and protects these biomolecules from the aggressive media of the wound.
可生物降解的纳米纤维广泛应用于生物学和医学的不同领域，尤其是组织工程领域。电纺聚己内酯（PCL）纳米纤维因其良好的机械性能和类似于细胞外基质的低成本结构而越来越受到关注。然而，未改性的PCL纳米纤维表面呈惰性，阻碍细胞黏附并对其后续命运产生负面影响。将PCL纳米纤维支架用于伤口愈合需要对PCL表面进行一定的改性。在这项工作中，通过仔细调整电纺参数优化了PCL纳米纤维的形态。结果表明，用COOH等离子体聚合物对PCL纳米纤维进行改性，随后结合蛋白质分子的NH基团，是一种相当简单且技术上可行的方法，可以促进人成纤维细胞的黏附、早期铺展和生长。与先前研究的间充质干细胞在PCL纳米纤维网上的培养相比，发现成纤维细胞在改性PCL表面的行为有很大不同。X射线光电子能谱（XPS）表明，冻融富血小板血浆（PRP）的固定可以通过共价键和非共价键进行，并且不会影响其生物活性。与未改性的PCL纳米纤维或通过PRP非共价键结合的PCL纳米纤维相比，PRP的共价结合成分可显著减少成纤维细胞凋亡并增加细胞增殖。报道的研究结果揭示了PCL基质在组织工程中的应用潜力，而用COOH基团进行等离子体改性并随后与蛋白质进行共价结合进一步扩大了这种潜力。使用这种共价固定PRP的基质进行伤口愈合可延长固定分子的生物活性，并保护这些生物分子免受伤口侵袭性介质的影响。
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