Arp L H, McDonald S M
Avian Dis. 1985 Oct-Dec;29(4):1066-77.
Effects of temperature on growth of three strains of Bordetella avium were determined in young turkeys and in vitro. Colonization of the tracheal mucosa by two virulent strains of B. avium was significantly greater in cold-stressed turkeys than in heat-stressed turkeys. The avirulent vaccine strain, ART-VAX, colonized tracheas of cold-stressed turkeys to a limited extent but failed to colonize heat-stressed turkeys. Growth rates of the three B. avium strains were determined in brain-heart infusion broth at 30, 35, 40, and 45 C. All three strains grew best at 35 C but were killed by 45 C. Compared with virulent strains, ART-VAX grew markedly less at all temperatures, and most cultures of ART-VAX grew at 40 C only after a variable period of declining numbers of viable bacteria. This study indicates that temperature affects growth of B. avium in vivo and in vitro and that growth of the ART-VAX strain is fundamentally different from growth of virulent strains.
在幼火鸡体内和体外测定了温度对三种禽博德特氏菌菌株生长的影响。两种强毒力禽博德特氏菌菌株在冷应激火鸡的气管黏膜上的定植显著多于热应激火鸡。无毒力疫苗株ART-VAX在冷应激火鸡的气管中有一定程度的定植，但未能在热应激火鸡的气管中定植。在30、35、40和45℃下，在脑心浸液肉汤中测定了三种禽博德特氏菌菌株的生长速率。所有三种菌株在35℃时生长最佳，但在45℃时均被杀死。与强毒株相比，ART-VAX在所有温度下的生长均明显较少，并且ART-VAX的大多数培养物仅在活菌数量经过一段可变的下降期后才在40℃下生长。本研究表明，温度影响禽博德特氏菌在体内和体外的生长，并且ART-VAX菌株的生长与强毒株的生长有根本差异。
