• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从人诱导多能干细胞中可重复且可扩展地分化出高纯度皮质神经元。

Reproducible and scalable differentiation of highly pure cortical neurons from human induced pluripotent stem cells.

作者信息

Dannert Angelika, Klimmt Julien, Cardoso Gonçalves Carolina, Crusius Dennis, Paquet Dominik

机构信息

Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, 81377 Munich, Germany; Graduate School of Systemic Neurosciences, LMU Munich, 81377 Munich, Germany.

Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, 81377 Munich, Germany.

出版信息

STAR Protoc. 2023 May 4;4(2):102266. doi: 10.1016/j.xpro.2023.102266.

DOI:10.1016/j.xpro.2023.102266
PMID:37148244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10193006/
Abstract

Human-induced-pluripotent-stem-cell (hiPSC)-derived neurons are valuable for investigating brain physiology and disease. Here, we present a protocol to differentiate hiPSCs into cortical neurons with high yield and purity. We describe neural induction via dual-SMAD inhibition, followed by spot-based differentiation to provide high quantities of neural precursors. We detail their enrichment, expansion, and purification to avoid unwanted cell fates and provide optimal conditions for neural rosette proliferation. These differentiated neurons are suitable for drug testing and co-culture studies. For complete details on the use and execution of this protocol, please refer to Paquet et al. and Weisheit et al...

摘要

人诱导多能干细胞（hiPSC）衍生的神经元对于研究脑生理学和疾病具有重要价值。在此，我们展示了一种将hiPSC高效、高纯度地分化为皮质神经元的方案。我们描述了通过双SMAD抑制进行神经诱导，随后进行基于斑点的分化以提供大量神经前体。我们详细介绍了它们的富集、扩增和纯化，以避免出现不需要的细胞命运，并为神经玫瑰花结增殖提供最佳条件。这些分化的神经元适用于药物测试和共培养研究。有关此方案的使用和执行的完整详细信息，请参考Paquet等人以及Weisheit等人的研究……

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/18e92d16811a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/d662b1344194/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/62846fe361a2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/5b6a747af244/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/e7c75d56c4b4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/bcb491485dc0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/18e92d16811a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/d662b1344194/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/62846fe361a2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/5b6a747af244/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/e7c75d56c4b4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/bcb491485dc0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/309b/10193006/18e92d16811a/gr5.jpg

相似文献

1
Reproducible and scalable differentiation of highly pure cortical neurons from human induced pluripotent stem cells.从人诱导多能干细胞中可重复且可扩展地分化出高纯度皮质神经元。
STAR Protoc. 2023 May 4;4(2):102266. doi: 10.1016/j.xpro.2023.102266.
2
A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes.基于微载体的规模化生产和纯化人诱导多能干细胞源性神经元和星形胶质细胞的方案。
STAR Protoc. 2022 Aug 18;3(3):101632. doi: 10.1016/j.xpro.2022.101632. eCollection 2022 Sep 16.
3
Generation of highly pure motor neurons from human induced pluripotent stem cells.从人诱导多能干细胞中生成高纯度运动神经元。
STAR Protoc. 2022 Mar 10;3(1):101223. doi: 10.1016/j.xpro.2022.101223. eCollection 2022 Mar 18.
4
Protocol for differentiation of functional macrophages from human induced pluripotent stem cells.分化人诱导多能干细胞为功能性巨噬细胞的方案。
STAR Protoc. 2024 Mar 15;5(1):102925. doi: 10.1016/j.xpro.2024.102925. Epub 2024 Feb 28.
5
Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons.优化的功能性人诱导多能干细胞源性多巴胺能神经元生成方案。
STAR Protoc. 2023 Sep 15;4(3):102486. doi: 10.1016/j.xpro.2023.102486. Epub 2023 Jul 28.
6
Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of fatty acid synthesis.通过抑制脂肪酸合成从人多能干细胞中纯化心肌细胞和神经元。
STAR Protoc. 2022 Apr 28;3(2):101360. doi: 10.1016/j.xpro.2022.101360. eCollection 2022 Jun 17.
7
Optimized protocol for analysis of neural stem proliferation in human-pluripotent-stem-cell-derived cerebral organoids.人多能干细胞来源的脑类器官中神经干细胞增殖分析的优化方案。
STAR Protoc. 2023 Mar 14;4(2):102169. doi: 10.1016/j.xpro.2023.102169.
8
Protocol for neurogenin-2-mediated induction of human stem cell-derived neural progenitor cells.神经母细胞瘤基因-2 介导的人干细胞源性神经祖细胞诱导方案。
STAR Protoc. 2024 Mar 15;5(1):102878. doi: 10.1016/j.xpro.2024.102878. Epub 2024 Feb 8.
9
Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids.从人诱导多能干细胞衍生的胰腺祖细胞类器官中分化出β样细胞。
STAR Protoc. 2022 Aug 30;3(3):101656. doi: 10.1016/j.xpro.2022.101656. eCollection 2022 Sep 16.
10
Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes.分化单层人类诱导多能干细胞为炎症反应性星形胶质细胞的方案。
STAR Protoc. 2023 Mar 17;4(1):102142. doi: 10.1016/j.xpro.2023.102142. Epub 2023 Mar 6.

引用本文的文献

1
Pulsed electromagnetic stimulation promotes neuronal maturation by up-regulating cholesterol biosynthesis.脉冲电磁刺激通过上调胆固醇生物合成来促进神经元成熟。
Stem Cell Res Ther. 2025 Jul 26;16(1):406. doi: 10.1186/s13287-025-04469-1.
2
Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation.用于衰老建模和基因操作的人胚胎干细胞衍生神经元的应用方案。
STAR Protoc. 2025 Mar 21;6(1):103633. doi: 10.1016/j.xpro.2025.103633. Epub 2025 Feb 10.
3
Cell-type-specific loops linked to RNA polymerase II elongation in human neural differentiation.

本文引用的文献

1
An epigenetic barrier sets the timing of human neuronal maturation.表观遗传屏障决定了人类神经元成熟的时间。
Nature. 2024 Feb;626(8000):881-890. doi: 10.1038/s41586-023-06984-8. Epub 2024 Jan 31.
2
A reference human induced pluripotent stem cell line for large-scale collaborative studies.一个用于大规模合作研究的参考性人诱导多能干细胞系。
Cell Stem Cell. 2022 Dec 1;29(12):1685-1702.e22. doi: 10.1016/j.stem.2022.11.004.
3
Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media.
人类神经分化中与 RNA 聚合酶 II 延伸相关的细胞类型特异性环。
Cell Genom. 2024 Aug 14;4(8):100606. doi: 10.1016/j.xgen.2024.100606. Epub 2024 Jul 10.
4
Tackling neurodegeneration with omics: a path towards new targets and drugs.借助组学技术应对神经退行性疾病：通往新靶点和新药物的道路。
Front Mol Neurosci. 2024 Jun 17;17:1414886. doi: 10.3389/fnmol.2024.1414886. eCollection 2024.
5
Accelerating Diverse Cell-Based Therapies Through Scalable Design.通过可扩展设计加速多样化的基于细胞的疗法。
Annu Rev Chem Biomol Eng. 2024 Jul;15(1):267-292. doi: 10.1146/annurev-chembioeng-100722-121610. Epub 2024 Jul 3.
6
A multi-looping chromatin signature predicts dysregulated gene expression in neurons with familial Alzheimer's disease mutations.一种多环染色质特征可预测携带家族性阿尔茨海默病突变的神经元中基因表达失调。
bioRxiv. 2024 Feb 27:2024.02.27.582395. doi: 10.1101/2024.02.27.582395.
7
Cell type-specific loops linked to RNA polymerase II elongation in human neural differentiation.与人类神经分化中RNA聚合酶II延伸相关的细胞类型特异性环。
bioRxiv. 2023 Dec 5:2023.12.04.569731. doi: 10.1101/2023.12.04.569731.
在化学成分确定的培养基中从人诱导多能干细胞中分化功能性星形胶质细胞。
STAR Protoc. 2021 Oct 20;2(4):100902. doi: 10.1016/j.xpro.2021.100902. eCollection 2021 Dec 17.
4
A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.一种多功能多药理学平台促进人类多能性和分化细胞的细胞保护和活力。
Nat Methods. 2021 May;18(5):528-541. doi: 10.1038/s41592-021-01126-2. Epub 2021 May 3.
5
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.HDR 介导的 CRISPR 编辑后有害的靶标效应的检测。
Cell Rep. 2020 May 26;31(8):107689. doi: 10.1016/j.celrep.2020.107689.
6
Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells.联合小分子抑制可加速从人多能干细胞中获得功能性皮质神经元。
Nat Biotechnol. 2017 Feb;35(2):154-163. doi: 10.1038/nbt.3777. Epub 2017 Jan 23.
7
Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9.利用 CRISPR/Cas9 高效引入特定的纯合子和杂合子突变。
Nature. 2016 May 5;533(7601):125-9. doi: 10.1038/nature17664. Epub 2016 Apr 27.
8
Rapid and robust generation of long-term self-renewing human neural stem cells with the ability to generate mature astroglia.能够生成成熟星形胶质细胞的长期自我更新人类神经干细胞的快速且强劲生成。
Sci Rep. 2015 Nov 6;5:16321. doi: 10.1038/srep16321.
9
Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks.人多能干细胞向大脑皮层神经元和神经网络的定向分化。
Nat Protoc. 2012 Oct;7(10):1836-46. doi: 10.1038/nprot.2012.116. Epub 2012 Sep 13.
10
Fiji: an open-source platform for biological-image analysis.斐济：一个用于生物影像分析的开源平台。
Nat Methods. 2012 Jun 28;9(7):676-82. doi: 10.1038/nmeth.2019.