Dong Wen, Yang Xin, Zhang Ning, Chen Pei, Sun Jianghao, Harnly James M, Zhang Mengliang
Department of Chemistry, Middle Tennessee State University, Murfreesboro, TN 37132, United States; Department of Computer Science, Middle Tennessee State University, Murfreesboro, TN 37132, United States.
Department of Computer Science, Middle Tennessee State University, Murfreesboro, TN 37132, United States.
Food Chem. 2024 Jun 30;444:138653. doi: 10.1016/j.foodchem.2024.138653. Epub 2024 Feb 5.
The effects of anthocyanin's substitution groups on the UV-Vis molar absorptivity were examined by analyzing a group of 31 anthocyanidin/anthocyanin reference standards with ultra-high performance liquid chromatography-diode array detector (UHPLC-DAD). The substitution groups on aglycones were found to associate with molar absorptivity variations, often neglected in anthocyanin quantitation, resulting in significant analytical errors. A simple yet comprehensive strategy based on the molar relative response factors (MRRFs) and a single master reference calibration (i.e., cyanidin-3-glucoside) was proposed to quantify anthocyanins in red cabbage, blueberry, and strawberry samples with improved analytical accuracy. The results indicate this approach provides an effective, inexpensive, and accurate analytical method for anthocyanins in food materials without using individual reference standards. MRRFs of 617 anthocyanins/anthocyanidins were calculated, and the information is freely available at https://BotanicalDC.online/anthocyanin/. This study could be critical to developing new reference methods for anthocyanin analysis and harmonizing results and data from various sources.
通过使用超高效液相色谱 - 二极管阵列检测器（UHPLC - DAD）分析一组31种花青素/花色苷参考标准品，研究了花青素取代基对紫外 - 可见摩尔吸光率的影响。发现苷元上的取代基与摩尔吸光率变化相关，而这在花青素定量中常常被忽视，从而导致显著的分析误差。提出了一种基于摩尔相对响应因子（MRRFs）和单一主参考校准（即矢车菊素 - 3 - 葡萄糖苷）的简单而全面的策略，以提高红甘蓝、蓝莓和草莓样品中花青素定量分析的准确性。结果表明，该方法为食品原料中的花青素提供了一种有效、廉价且准确的分析方法，无需使用单个参考标准品。计算了617种花青素/花色苷的MRRFs，相关信息可在https://BotanicalDC.online/anthocyanin/免费获取。本研究对于开发新的花青素分析参考方法以及统一来自各种来源的结果和数据可能至关重要。
