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香蕉中响应与株高相关的赤霉素和独脚金内酯的基因的全基因组鉴定与表达分析

Genome-Wide Identification and Expression Analysis of Gene in Response to GA and SL Related to Plant Height in Banana.

作者信息

Tong Ning, Zhang Chunyu, Xu Xiaoqiong, Zhang Zhilin, Li Jiahui, Liu Zhaoyang, Chen Yukun, Zhang Zihao, Huang Yuji, Lin Yuling, Lai Zhongxiong

机构信息

Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

出版信息

Plants (Basel). 2024 Feb 5;13(3):458. doi: 10.3390/plants13030458.

DOI:10.3390/plants13030458
PMID:38337990
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10857657/
Abstract

Dwarfing is one of the common phenotypic variations in asexually reproduced progeny of banana, and dwarfed banana is not only windproof and anti-fallout but also effective in increasing acreage yield. As a key gene in the strigolactone signalling pathway, () plays an important role in the regulation of the height of plants. In order to gain insight into the function of the banana gene, this study conducted genome-wide identification of banana gene based on the , and genome database. Analysis of gene expression under high temperature, low temperature and osmotic stress based on transcriptome data and RT-qPCR was used to analyse gene expression in different tissues as well as in different concentrations of GA and SL treatments. In this study, we identified three , three and two genes in banana. Phylogenetic tree analysis showed that D53 Musa are equally related to D53 Asparagales and Poales. Both high and low-temperature stresses substantially reduced the expression of the gene, but osmotic stress treatments had less effect on the expression of the gene. GR24 treatment did not significantly promote the height of the banana, but the expression of the gene was significantly reduced in roots and leaves. GA treatment at 100 mg/L significantly promoted the expression of the gene in roots, but the expression of this gene was significantly reduced in leaves. In this study, we concluded that responds to GA and SL treatments, but "Yinniaijiao" dwarf banana may not be sensitive to GA and SL.

摘要

矮化是香蕉无性繁殖后代常见的表型变异之一,矮化香蕉不仅抗风抗倒伏,还能有效提高单位面积产量。作为独脚金内酯信号通路中的关键基因,()在调控植物株高方面发挥着重要作用。为深入了解香蕉基因的功能,本研究基于、和基因组数据库对香蕉基因进行了全基因组鉴定。基于转录组数据以及RT-qPCR分析了高温、低温和渗透胁迫下基因的表达情况,以分析该基因在不同组织以及不同浓度赤霉素(GA)和独脚金内酯(SL)处理下的表达。在本研究中,我们在香蕉中鉴定出了3个、3个和2个基因。系统发育树分析表明,香蕉的D53与天门冬目和禾本目的D53亲缘关系相当。高温和低温胁迫均显著降低了基因的表达,但渗透胁迫处理对该基因表达的影响较小。GR24处理未显著促进香蕉株高增长,但基因在根和叶中的表达显著降低。100 mg/L的GA处理显著促进了该基因在根中的表达,但该基因在叶中的表达显著降低。在本研究中,我们得出结论,对GA和SL处理有响应,但“银矮蕉”矮化香蕉可能对GA和SL不敏感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/ce3e77112e39/plants-13-00458-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/c35048acc19e/plants-13-00458-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/309b6162a6fa/plants-13-00458-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/5ef13b51719a/plants-13-00458-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/395580c7dfa7/plants-13-00458-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/130cc8f04066/plants-13-00458-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/531ffbaaceea/plants-13-00458-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/0c148b46f8a3/plants-13-00458-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/ce3e77112e39/plants-13-00458-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/c35048acc19e/plants-13-00458-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/309b6162a6fa/plants-13-00458-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/5ef13b51719a/plants-13-00458-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/395580c7dfa7/plants-13-00458-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/130cc8f04066/plants-13-00458-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/531ffbaaceea/plants-13-00458-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/0c148b46f8a3/plants-13-00458-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/10857657/ce3e77112e39/plants-13-00458-g008.jpg

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