Zhao Penghui, Hou Yinchen, Chen Xinyang, Zhang Mingyi, Hu Zheyuan, Chen Lishui, Huang Jihong
Food Laboratory of Zhong Yuan, Luohe 462300, China.
School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Foods. 2024 Jan 29;13(3):436. doi: 10.3390/foods13030436.
High Fischer ratio oligopeptides (HFOs) exhibit diverse biological activities, including anti-inflammatory and antioxidant properties. HFOs from gluten origin were prepared through fermentation and enzymatic hydrolysis and then characterized using free amino acid analysis and scanning electron microscopy (SEM). Following intervention, the levels of serum total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatic malondialdehyde (MDA) in the rats significantly decreased ( < 0.05). Simultaneously, there was an increasing trend in superoxide dismutase (SOD) levels, and glutathione (GSH) levels were significantly elevated ( < 0.05). The mRNA expression levels of alcohol metabolism-related genes (ADH4, ALDH2, and CYP2E1) exhibited a significant increase ( < 0.05). Histological examination revealed a reduction in liver damage. The findings indicate that high Fischer ratio oligopeptides, prepared through enzymatic and fermentation methods, significantly improve lipid levels, ameliorate lipid metabolism disorders, and mitigate oxidative stress, and exhibit a discernible alleviating effect on alcoholic liver injury in rats.
高Fischer比值寡肽（HFOs）具有多种生物活性，包括抗炎和抗氧化特性。通过发酵和酶解制备了源于麸质的HFOs，然后利用游离氨基酸分析和扫描电子显微镜（SEM）对其进行表征。干预后，大鼠血清总胆固醇（TC）、甘油三酯（TG）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和肝脏丙二醛（MDA）水平显著降低（<0.05）。同时，超氧化物歧化酶（SOD）水平呈上升趋势，谷胱甘肽（GSH）水平显著升高（<0.05）。酒精代谢相关基因（ADH4、ALDH2和CYP2E1）的mRNA表达水平显著增加（<0.05）。组织学检查显示肝损伤减轻。研究结果表明，通过酶法和发酵法制备的高Fischer比值寡肽可显著改善血脂水平，改善脂质代谢紊乱，减轻氧化应激，并对大鼠酒精性肝损伤具有明显的缓解作用。
