Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
National and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.
Mar Drugs. 2024 Jul 19;22(7):322. doi: 10.3390/md22070322.
High Fischer ratio oligopeptides (HFOs) have a variety of biological activities, but their mechanisms of action for anti-fatigue are less systematically studied at present. This study aimed to systematically evaluate the anti-fatigue efficacy of HFOs from Antarctic krill (HFOs-AK) and explore its mechanism of action through establishing the fatigue model of endurance swimming in mice. Therefore, according to the comparison with the endurance swimming model group, HFOs-AK were able to dose-dependently prolong the endurance swimming time, reduce the levels of the metabolites (lactic acid, blood urea nitrogen, and blood ammonia), increase the content of blood glucose, muscle glycogen, and liver glycogen, reduce lactate dehydrogenase and creatine kinase extravasation, and protect muscle tissue from damage in the endurance swimming mice. HFOs-AK were shown to enhance Na-K-ATPase and Ca-Mg-ATPase activities and increase ATP content in muscle tissue. Meanwhile, HFOs-AK also showed significantly antioxidant ability by increasing the activities of superoxide dismutase and glutathione peroxidase in the liver and decreasing the level of malondialdehyde. Further studies showed that HFOs-AK could regulate the body's energy metabolism and thus exert its anti-fatigue effects by activating the AMPK signaling pathway and up-regulating the expression of p-AMPK and PGC-α proteins. Therefore, HFOs-AK can be used as an auxiliary functional dietary molecules to exert its good anti-fatigue activity and be applied to anti-fatigue functional foods.
高 Fischer 比寡肽（HFOs）具有多种生物活性，但目前其抗疲劳作用机制的研究还不够系统。本研究旨在通过建立小鼠耐力游泳疲劳模型，系统评价南极磷虾 HFOs（HFOs-AK）的抗疲劳功效，并探讨其作用机制。因此，与耐力游泳模型组相比，HFOs-AK 能够剂量依赖性地延长耐力游泳时间，降低代谢产物（乳酸、血尿素氮和血氨）水平，增加血糖、肌肉糖原和肝糖原含量，减少乳酸脱氢酶和肌酸激酶渗出，保护耐力游泳小鼠的肌肉组织免受损伤。HFOs-AK 可增强 Na-K-ATP 酶和 Ca-Mg-ATP 酶的活性，并增加肌肉组织中的 ATP 含量。同时，HFOs-AK 还通过增加肝中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性以及降低丙二醛的水平，表现出显著的抗氧化能力。进一步的研究表明，HFOs-AK 通过激活 AMPK 信号通路和上调 p-AMPK 和 PGC-α 蛋白的表达，调节机体的能量代谢，从而发挥其抗疲劳作用。因此，HFOs-AK 可以作为一种辅助功能性膳食分子，发挥其良好的抗疲劳活性，并应用于抗疲劳功能性食品。
