National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China.
Int J Mol Sci. 2024 Feb 5;25(3):1898. doi: 10.3390/ijms25031898.
Growth is a fundamental aspect of aquaculture breeding programs, pivotal for successful cultivation. Understanding the mechanisms that govern growth and development differences across various stages can significantly boost seedling production of economically valuable species, thereby enhancing aquaculture efficiency and advancing the aquaculture industry. , a commercially vital marine bivalve, underscores this importance. To decipher the intricate molecular mechanisms dictating growth and developmental disparities in marine shellfish, we conducted transcriptome sequencing and meticulously analyzed gene expression variations and molecular pathways linked to growth traits in . This study delved into the molecular and gene expression variations across five larval development stages, with a specific focus on scrutinizing the differential expression patterns of growth-associated genes using RNA sequencing and quantitative real-time PCR analysis. A substantial number of genes-36,044 differentially expressed genes (DEGs)-exhibited significant differential expression between consecutive developmental stages. These DEGs were then categorized into multiple pathways (Q value < 0.05), including crucial pathways such as the spliceosome, vascular smooth muscle contraction, DNA replication, and apoptosis, among others. In addition, we identified two pivotal signaling pathways-the Hedgehog (Hh) signaling pathway and the TGF-beta (TGF-β) signaling pathway-associated with the growth and development of larvae. Ten key growth-related genes were pinpointed, each playing crucial roles in molecular function and the regulation of growth traits in . These genes and pathways associated with growth provide deep insights into the molecular basis of physiological adaptation, metabolic processes, and growth variability in marine bivalves.
生长是水产养殖繁殖计划的一个基本方面，对于成功的养殖至关重要。了解控制不同阶段生长和发育差异的机制，可以显著提高经济价值物种的苗种产量，从而提高水产养殖效率，推动水产养殖业的发展。作为一种商业上重要的海洋双壳贝类，强调了这一点的重要性。为了解密控制海洋贝类生长和发育差异的复杂分子机制，我们进行了转录组测序，并细致分析了与生长特征相关的基因表达变化和分子途径。本研究深入探讨了五个幼虫发育阶段的分子和基因表达变化，特别关注使用 RNA 测序和定量实时 PCR 分析研究与生长相关的基因的差异表达模式。大量基因-36044 个差异表达基因（DEGs）-在连续的发育阶段之间表现出显著的差异表达。然后，这些 DEGs 被归类为多个途径（Q 值<0.05），包括剪接体、血管平滑肌收缩、DNA 复制和细胞凋亡等关键途径。此外，我们还鉴定了与幼虫生长和发育相关的两个重要信号通路- Hedgehog（Hh）信号通路和 TGF-β（TGF-β）信号通路。确定了 10 个关键的生长相关基因，每个基因在 分子功能和生长特征的调控中都起着关键作用。这些与生长相关的基因和途径为海洋双壳贝类的生理适应、代谢过程和生长变异性提供了深入的分子基础。
