National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316004, China.
National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316004, China.
Fish Shellfish Immunol. 2017 Nov;70:560-567. doi: 10.1016/j.fsi.2017.08.034. Epub 2017 Aug 30.
As an economically important bivalve, the Mytilus coruscus is cultured widely in the eastern coast of China. In recent years, this bivalve has been seriously affected by the pathogenic infections. To elucidate the host defense mechanisms of M. coruscus against pathogenic challenge, the hemocyte transcriptomes of M. coruscus before and after Vibrio alginnolyficus infection were analyzed using the deep-sequencing platform Illumina/HiSeq-2500, meanwhile the differentially expressed genes (DEGs) were investigated. In total, 130,031,083 clean reads were obtained and then assembled into 63,942 unigenes with an average length of 810 bp and an N50 of 1056 bp. Unigenes were annotated by comparing against nr, Swiss-Prot, KEGG, COG, KOG, GO, and Pfam databases, and 27,345 unigenes (42.77%) were annotated in at least one database. After bacterial challenge, 1270 and 265 genes were identified as remarkably up- or down-regulated, respectively, amongst 1154 were associated with 122 pathways, including classical immune-related pathways, such as 'Toll-like receptor signaling', 'the complement cascades', 'MAPK signaling pathway', 'Apoptosis' and 'Wnt signaling pathway'. Besides, nine genes which were differently-expressed immuno-related were confirmed by using quantitative real-time PCR. These findings would provide new insights on the M. coruscus innate immunity, based on which, some novel strategies for management of diseases and long-term sustainability of M. coruscus culture could be developed.
作为一种具有重要经济价值的双壳贝类，贻贝在中国东部广泛养殖。近年来，这种贝类受到了致病性感染的严重影响。为了阐明贻贝抵御病原感染的宿主防御机制，我们利用 Illumina/HiSeq-2500 高通量测序平台分析了贻贝在感染 Alginnolyticus 弧菌前后的血细胞转录组，并研究了差异表达基因（DEGs）。总共获得了 130,031,083 条清洁reads，然后组装成 63,942 个 unigenes，平均长度为 810bp，N50 为 1056bp。通过与 nr、Swiss-Prot、KEGG、COG、KOG、GO 和 Pfam 数据库进行比对，对 unigenes 进行了注释，其中 27,345 个 unigenes（42.77%）在至少一个数据库中得到注释。在细菌感染后，分别有 1270 个和 265 个基因被鉴定为显著上调或下调，其中 1154 个基因与 122 条途径相关，包括经典的免疫相关途径，如“Toll-like receptor signaling”、“the complement cascades”、“MAPK signaling pathway”、“Apoptosis”和“Wnt signaling pathway”。此外，通过定量实时 PCR 验证了 9 个差异表达的免疫相关基因。这些发现为贻贝先天免疫提供了新的见解，在此基础上，可以开发一些新的疾病管理策略和贻贝养殖的长期可持续性策略。
