Division of Biotechnology and Molecular Medicine, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA.
Virus Genes. 2024 Apr;60(2):148-158. doi: 10.1007/s11262-024-02054-x. Epub 2024 Feb 10.
Guinea Pig Herpes-Like Virus (GPHLV) is a virus isolated from leukemic guinea pigs with herpes virus-like morphology described by Hsiung and Kaplow in 1969. GPHLV transformed embryonic cells from Syrian hamsters or rats, which were tumorigenic in adult animals. Herein, we present the genomic sequence of GPHLV strain LK40 as a reference for future molecular analysis. GPHLV has a broad host tropism and replicates efficiently in Guinea pig, Cat, and Green African Monkey-derived cell lines. GPHLV has a GC content of 35.45%. The genome is predicted to encode at least 75 open-reading frames (ORFs) with 84% (63 ORFs) sharing homology to human Kaposi Sarcoma Associated Herpes Virus (KSHV). Importantly, GPHLV encodes homologues of the KSHV oncogenes, vBCL2 (ORF16), vPK (ORF36), viral cyclin (v-cyclin, ORF72), the latency associated nuclear antigen (LANA, ORF73), and vGPCR (ORF74). GPHLV is a Rhadinovirus of Cavia porcellus, and we propose the formal name of Caviid gamma herpesvirus 1 (CaGHV-1). GPHLV can be a novel small animal model of Rhadinovirus pathogenesis with broad host tropism.
豚鼠疱疹样病毒（GPHLV）是一种 1969 年由 Hsiung 和 Kaplow 从白血病豚鼠中分离出的具有疱疹病毒样形态的病毒。GPHLV 可转化叙利亚仓鼠或大鼠的胚胎细胞，这些细胞在成年动物中具有致瘤性。在此，我们提供了 GPHLV 株 LK40 的基因组序列，作为未来分子分析的参考。GPHLV 宿主范围广泛，可在豚鼠、猫和绿猴源性细胞系中高效复制。GPHLV 的 GC 含量为 35.45%。基因组预测至少编码 75 个开放阅读框（ORFs），其中 84%（63 个 ORFs）与人类卡波济肉瘤相关疱疹病毒（KSHV）具有同源性。重要的是，GPHLV 编码了 KSHV 癌基因的同源物，如 vBCL2（ORF16）、vPK（ORF36）、病毒周期蛋白（v-cyclin，ORF72）、潜伏相关核抗原（LANA，ORF73）和 vGPCR（ORF74）。GPHLV 是豚鼠的 Rhadinovirus，我们提议正式名称为 Caviid gamma 疱疹病毒 1（CaGHV-1）。GPHLV 可能是一种具有广泛宿主范围的新型 Rhadinovirus 发病机制的小动物模型。
