Gonzalez-Serva A, Hsiung G D
Am J Pathol. 1978 Jun;91(3):483-96.
The role of bone marrow adherent cells in the latency of guinea pig herpes-like virus (GPHLV) was explored. Cultures of macrophage-enriched adherent cells derived from infected guinea pigs were examined for evidence of latent GPHLV infection. Expression of the virus was detected in these cultures 9 to 10 days after in vitro cultivation. Increasing virus infectivity titers as well as light and electron microscopic evidence of virion assembly in macrophages and fibroblasts were demonstrated. Infections virus was detected in the bone marrow adherent cells that had attached for 30 or 120 minutes but only following reverse cocultivation. The data showed not only that the bone marrow adherent cells were susceptible to GPHLV in vitro but also that GPHLV was harbored by the macrophage-enriched bone marrow population in vivo in latently infected guinea pigs.
探讨了骨髓黏附细胞在豚鼠类疱疹病毒（GPHLV）潜伏中的作用。对来自感染豚鼠的富含巨噬细胞的黏附细胞培养物进行检测，以寻找潜伏性GPHLV感染的证据。在体外培养9至10天后，在这些培养物中检测到病毒表达。证明了病毒感染性滴度增加以及在巨噬细胞和成纤维细胞中病毒粒子组装的光镜和电镜证据。在附着30或120分钟的骨髓黏附细胞中检测到感染性病毒，但仅在反向共培养后。数据表明，骨髓黏附细胞在体外对GPHLV敏感，而且在潜伏感染的豚鼠体内，富含巨噬细胞的骨髓群体中存在GPHLV。
