Reiter Michael A, Bradley Timothy, Büchel Lars A, Keller Philipp, Hegedis Emese, Gassler Thomas, Vorholt Julia A
Institute of Microbiology, Department of Biology, ETH Zurich, Zurich, Switzerland.
Nat Catal. 2024;7(5):560-573. doi: 10.1038/s41929-024-01137-0. Epub 2024 Apr 23.
Methanol synthesized from captured greenhouse gases is an emerging renewable feedstock with great potential for bioproduction. Recent research has raised the prospect of methanol bioconversion to value-added products using synthetic methylotrophic , as its metabolism can be rewired to enable growth solely on the reduced one-carbon compound. Here we describe the generation of an strain that grows on methanol at a doubling time of 4.3 h-comparable to many natural methylotrophs. To establish bioproduction from methanol using this synthetic chassis, we demonstrate biosynthesis from four metabolic nodes from which numerous bioproducts can be derived: lactic acid from pyruvate, polyhydroxybutyrate from acetyl coenzyme A, itaconic acid from the tricarboxylic acid cycle and -aminobenzoic acid from the chorismate pathway. In a step towards carbon-negative chemicals and valorizing greenhouse gases, our work brings synthetic methylotrophy in within reach of industrial applications.
由捕获的温室气体合成的甲醇是一种新兴的可再生原料，在生物生产方面具有巨大潜力。最近的研究提出了利用合成甲基营养菌将甲醇生物转化为增值产品的前景，因为其新陈代谢可以重新调整，使其仅依靠还原态的一碳化合物生长。在此，我们描述了一种菌株的产生，该菌株能以4.3小时的倍增时间在甲醇上生长，这与许多天然甲基营养菌相当。为了利用这种合成底盘从甲醇中进行生物生产，我们展示了从四个代谢节点进行的生物合成，从这些节点可以衍生出许多生物产品：由丙酮酸生成乳酸、由乙酰辅酶A生成聚羟基丁酸酯、由三羧酸循环生成衣康酸以及由分支酸途径生成对氨基苯甲酸。朝着碳负化学品和温室气体增值迈出的一步，我们的工作使工业应用中的合成甲基营养成为可能。
