Zhang Jingqing, Chen Yuxu, Xu Yuanyu, Zhao Zhimin, Xu Xinjun
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Pharmaceutics. 2025 Apr 18;17(4):532. doi: 10.3390/pharmaceutics17040532.
Silver nanoparticles (AgNPs) were synthesized via an easy and rapid biogenic synthesis approach, utilizing the dual capabilities of salicylic acid as both a reducing and capping agent. The characterization of Salicylic Acid-Mediated Silver Nanoparticle (SA-AgNPs) was conducted using a variety of techniques, including ultraviolet-visible spectroscopy, dynamic light scattering, scanning electron microscopy combined with energy dispersive X-ray spectroscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, as well as thermogravimetric analysis paired with differential scanning calorimetry. SA-AgNPs demonstrated significant antibacterial properties against both Gram-positive (methicillin-resistant , , , methicillin-resistant ) and Gram-negative (), with minimum inhibitory concentrations (MICs) of 8, 9, 8, 4, and 6 μg/mL, respectively. At a concentration of 32 μg/mL, SA-AgNPs exhibited 99.9% killing efficiency against (), (), and methicillin-resistant (MRSA), within 4, 16, and 12 h, respectively. At the same concentration, SA-AgNPs effectively inhibited 95.61% of MRSA biofilm formation. SA-AgNPs induced the leakage of intracellular macromolecular substances by increasing the membrane permeability, which ultimately caused bacterial apoptosis. Overall, this study presents a fast and environmentally friendly approach for synthesizing SA-AgNPs, with potential applications as nano antibiotics antibacterial coatings for implantable medical devices and wound dressings.
通过一种简单快速的生物合成方法合成了银纳米颗粒（AgNPs），利用了水杨酸作为还原剂和封端剂的双重能力。使用多种技术对水杨酸介导的银纳米颗粒（SA-AgNPs）进行了表征，包括紫外可见光谱、动态光散射、扫描电子显微镜结合能量色散X射线光谱、透射电子显微镜、X射线衍射、傅里叶变换红外光谱，以及热重分析与差示扫描量热法联用。SA-AgNPs对革兰氏阳性菌（耐甲氧西林金黄色葡萄球菌、表皮葡萄球菌、溶血葡萄球菌、耐甲氧西林表皮葡萄球菌）和革兰氏阴性菌（大肠杆菌）均表现出显著的抗菌性能，最低抑菌浓度（MICs）分别为8、9、8、4和6μg/mL。在32μg/mL的浓度下，SA-AgNPs分别在4、16和12小时内对金黄色葡萄球菌、大肠杆菌和耐甲氧西林金黄色葡萄球菌（MRSA）的杀灭效率达到99.9%。在相同浓度下，SA-AgNPs有效抑制了95.61%的MRSA生物膜形成。SA-AgNPs通过增加膜通透性诱导细胞内大分子物质泄漏，最终导致细菌凋亡。总体而言，本研究提出了一种快速且环保的合成SA-AgNPs的方法，具有作为可植入医疗设备和伤口敷料的纳米抗生素抗菌涂层的潜在应用。
