Kurt-Celep İnci, Zengin Gokhan, Uba Abdullahi I, Caprioli Giovanni, Mustafa Ahmed M, Angeloni Simone, Cakilcioglu Ugur, Guler Osman, Kaplan Alevcan, Sharmeen Jugreet, Mahomoodally Mohamad F
Faculty of Pharmacy, Department of Pharmacognosy, Ataşehir, Acıbadem Mehmet Ali Aydınlar University, İstanbul, Turkey.
Department of Biology, Science Faculty, Selcuk University, Konya, Turkey.
Arch Pharm (Weinheim). 2023 Sep;356(9):e2300263. doi: 10.1002/ardp.202300263. Epub 2023 Jul 11.
Six extracts (water, ethanol, ethanol-water, ethyl acetate, dichloromethane, and n-hexane) of Astragalus caraganae were studied for their biological activities and bioactive contents. Based on high-performance liquid chromatography-mass spectrometry (HPLC-MS), the ethanol-water extract yielded the highest total bioactive content (4242.90 µg g ), followed by the ethanol and water extracts (3721.24 and 3661.37 µg g , respectively), while the least total bioactive content was yielded by the hexane extract, followed by the dichloromethane and ethyl acetate extracts (47.44, 274.68, and 688.89 µg g , respectively). Rutin, p-coumaric, chlorogenic, isoquercitrin, and delphindin-3,5-diglucoside were among the major components. Unlike the dichloromethane extracts, all the other extracts showed radical scavenging ability in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay (8.73-52.11 mg Trolox equivalent [TE]/g), while all extracts displayed scavenging property in the 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging assay (16.18-282.74 mg TE/g). The extracts showed antiacetylcholinesterase (1.27-2.73 mg galantamine equivalent [GALAE]/g), antibutyrylcholinesterase (0.20-5.57 mg GALAE/g) and antityrosinase (9.37-63.56 mg kojic acid equivalent [KAE]/g) effects. The molecular mechanism of the H O -induced oxidative stress pathway was aimed to be elucidated by applying ethanol, ethanol/water and water extracts at 200 µg/mL concentration to human dermal cells (HDFs). A. caraganae in HDF cells had neither a cytotoxic nor genotoxic effect but could have a cytostatic effect in increasing concentrations. The findings have allowed a better insight into the pharmacological potential of the plant, with respect to their chemical entities and bioactive contents, as well as extraction solvents and their polarity.
对柠条黄芪的六种提取物（水、乙醇、乙醇 - 水、乙酸乙酯、二氯甲烷和正己烷）进行了生物活性和生物活性成分研究。基于高效液相色谱 - 质谱联用（HPLC - MS）分析，乙醇 - 水提取物的总生物活性成分含量最高（4242.90μg/g），其次是乙醇提取物和水提取物（分别为3721.24和3661.37μg/g），而正己烷提取物的总生物活性成分含量最低，其次是二氯甲烷提取物和乙酸乙酯提取物（分别为47.44、274.68和688.89μg/g）。芦丁、对香豆酸、绿原酸、异槲皮苷和飞燕草素 - 3,5 - 二葡萄糖苷是主要成分。与二氯甲烷提取物不同，在2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基清除试验中，所有其他提取物均表现出自由基清除能力（8.73 - 52.11mg Trolox当量[TE]/g），而在2,2 - 联氮 - 双 - （3 - 乙基苯并噻唑啉 - 6 - 磺酸）（ABTS）自由基清除试验中，所有提取物均表现出清除特性（16.18 - 282.74mg TE/g）。提取物表现出抗乙酰胆碱酯酶（1.27 - 2.73mg加兰他敏当量[GALAE]/g）、抗丁酰胆碱酯酶（0.20 - 5.57mg GALAE/g）和抗酪氨酸酶（9.37 - 63.56mg曲酸当量[KAE]/g）作用。通过将浓度为200μg/mL的乙醇提取物、乙醇/水提取物和水提取物应用于人类皮肤成纤维细胞（HDFs），旨在阐明H₂O₂诱导的氧化应激途径的分子机制。柠条黄芪提取物对HDF细胞既无细胞毒性也无遗传毒性，但在浓度增加时可能具有细胞生长抑制作用。这些发现有助于更深入地了解该植物在化学实体、生物活性成分、提取溶剂及其极性方面的药理潜力。
