Gaviria G Yhoan S, Zapata M José E
Grupo de Investigación en Nutrición y Tecnología de Alimentos, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia.
Heliyon. 2023 May 12;9(5):e16165. doi: 10.1016/j.heliyon.2023.e16165. eCollection 2023 May.
Although the best-known use of the different species of earthworm is the production of organic fertilizer, they can also be considered a rich source of biologically and pharmacologically-active compounds, for use in the treatment of various diseases. In recent decades, with the development of biochemical technologies, research on the pharmaceutical effects of compounds extracted from different species of earthworms has begun. Enzymatic hydrolysis is the most common and widely used technique for producing bioactive hydrolysates, because it uses moderate operating conditions, with a certain specificity for the substrate. In the present study, the objective was to optimize and scale up the enzymatic hydrolysis of protein to obtain peptides with biological activity. The substrate characterization was carried out according to AOAC, a response surface design was performed for the optimization of the enzymatic hydrolysis and then the scaling was performed by means of dimensional analysis. The results show that the major component of the paste is protein, 65% of which is albumin, and the absence of pathogenic microorganisms was also found. Regarding optimization, it was found that the optimal hydrolysis conditions are achieved with pH = 8.5; temperature = 45 °C; amount of substrate = 125 g and volume of enzyme = 1245μL. For the scaling, 4 dimensionless pi-numbers were calculated which describe the process with no statistically significant differences between the model and the prototype; it can be concluded that the enzymatic hydrolysate of presents high values of antioxidant activity evaluated by different methodologies.
尽管蚯蚓不同种类最为人知的用途是生产有机肥料，但它们也可被视为生物活性和药理活性化合物的丰富来源，用于治疗各种疾病。近几十年来，随着生化技术的发展，对从不同种类蚯蚓中提取的化合物的药理作用的研究已经开始。酶水解是生产生物活性水解产物最常见和广泛使用的技术，因为它使用温和的操作条件，对底物具有一定的特异性。在本研究中，目标是优化并扩大蛋白质的酶水解以获得具有生物活性的肽。根据美国官方分析化学师协会（AOAC）进行底物表征，进行响应面设计以优化酶水解，然后通过量纲分析进行放大。结果表明，糊状物的主要成分是蛋白质，其中65%是白蛋白，并且还发现不存在致病微生物。关于优化，发现pH = 8.5、温度 = 45°C、底物量 = 125 g和酶体积 = 1245μL时可实现最佳水解条件。对于放大，计算了4个无量纲π数，它们描述了该过程，模型与原型之间无统计学显著差异；可以得出结论，通过不同方法评估，该酶水解产物具有高抗氧化活性值。
