Research Center for Neutrophil Engineering Technology, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
Cell Transplant. 2024 Jan-Dec;33:9636897241228031. doi: 10.1177/09636897241228031.
The management of granulocytopenia-associated infections is challenging, and a high mortality rate is associated with traditional supportive therapies. Neutrophils-the primary defenders of the human immune system-have potent bactericidal capabilities. Here, we investigated the dynamic in vivo distribution of neutrophil transfusion and their impact on the treatment outcome of severe granulocytopenic infections. We transfused Zr-labeled neutrophils in the C57BL/6 mice and observed the dynamic neutrophil distribution in mice for 24 h using the micro-positron emission tomography (Micro-PET) technique. The labeled neutrophils were predominantly retained in the lungs and spleen up to 4 h after injection and then redistributed to other organs, such as the spleen, liver, and bone marrow. Neutrophil transfusion did not elicit marked inflammatory responses or organ damage in healthy host mice. Notably, allogeneic neutrophils showed rapid chemotaxis to the infected area of the host within 1 h. Tail vein infusion of approximately 10 neutrophils substantially bolstered host immunity, ameliorated the inflammatory state, and increased survival rates in neutrophil-depleted and infected mice. Overall, massive allogeneic neutrophil transfusion had a therapeutic effect in severe infections and can have extensive applications in the future.
粒细胞减少相关感染的管理具有挑战性，传统的支持性治疗与高死亡率相关。中性粒细胞是人体免疫系统的主要防御细胞，具有强大的杀菌能力。在这里，我们研究了中性粒细胞输注的体内动态分布及其对严重粒细胞减少性感染治疗结果的影响。我们在 C57BL/6 小鼠中输注了 Zr 标记的中性粒细胞，并使用微正电子发射断层扫描（Micro-PET）技术观察了 24 小时内小鼠中中性粒细胞的动态分布。标记的中性粒细胞在注射后 4 小时内主要保留在肺部和脾脏中，然后重新分布到其他器官，如脾脏、肝脏和骨髓。中性粒细胞输注在健康宿主小鼠中不会引起明显的炎症反应或器官损伤。值得注意的是，同种异体中性粒细胞在 1 小时内迅速向宿主感染区域趋化。尾静脉输注约 10 个中性粒细胞可显著增强宿主免疫、改善炎症状态，并提高中性粒细胞耗竭和感染小鼠的存活率。总的来说，大量同种异体中性粒细胞输注对严重感染具有治疗作用，未来可能有广泛的应用。
