16S rRNA 测序鉴定胆道闭锁婴儿肠道微生物群的改变。
Alterations of gut microbiota in infants with biliary atresia identified by 16S rRNA-sequencing.
机构信息
Department of Pediatric Surgery, Guangdong Provincial Clinical Research Center for Child Health, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9 Jinsui Road, Guangzhou, Guangdong, 510623, China.
Department of Children Health Care, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9 Jinsui Road, Guangzhou, Guangdong, 510623, China.
出版信息
BMC Pediatr. 2024 Feb 14;24(1):117. doi: 10.1186/s12887-024-04582-9.
BACKGROUND
Biliary atresia (BA) is a severe neonatal disease with progressive intra- and extra-hepatic bile ducts inflammation and hepatic fibrosis. Characterization of gut microbiome profiles in infants with biliary atresia can provide valuable information and potential disease biomarkers. Our study aims to explore the relationship between gut microbiota and biliary atresia.
METHODS
16 S ribosomal RNA (rRNA) gene sequencing was carried out to identify the differences in composition and diversity of gut microbiota between infants with BA and healthy subjects. A total of 31 infants with biliary atresia and 20 healthy subjects were recruited.
RESULTS
The composition of gut microbiota in BA group was significantly different with the normal control group (P < 0.05) and the abundance ratio of Klebsiella/Bifidobacterium showed great potential for identification of BA (P < 0.01). In addition, the differential bacterial taxa were involved in lipid and vitamins metabolism.
CONCLUSION
Our results could provide potential non-invasive biomarker for identification of biliary atresia and contribute to the treatment in terms of ameliorating microbiota dysbiosis.
背景
胆道闭锁（BA）是一种严重的新生儿疾病，伴有进行性肝内外胆管炎症和肝纤维化。对胆道闭锁婴儿肠道微生物组谱的特征进行分析可以提供有价值的信息和潜在的疾病生物标志物。本研究旨在探讨肠道微生物群与胆道闭锁之间的关系。
方法
采用 16S 核糖体 RNA（rRNA）基因测序技术，比较胆道闭锁患儿与健康对照婴儿肠道微生物群落组成和多样性的差异。共纳入 31 例胆道闭锁患儿和 20 例健康对照婴儿。
结果
胆道闭锁患儿肠道微生物群落组成与正常对照组存在显著差异（P < 0.05），其中克雷伯氏菌/双歧杆菌的丰度比值对胆道闭锁的识别具有很大的潜力（P < 0.01）。此外，差异细菌类群参与脂质和维生素代谢。
结论
本研究结果可为胆道闭锁的识别提供潜在的非侵入性生物标志物，并有助于通过改善肠道微生物失调来改善治疗效果。
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