Department of Pediatrics, Hebei Medical University Third Hospital, Shijiazhuang, China.
Office of Academic Research, Beijing Children's Hospital Affiliated to Capital Medical University, Beijing, China.
Front Cell Infect Microbiol. 2024 Jun 13;14:1402329. doi: 10.3389/fcimb.2024.1402329. eCollection 2024.
In infants with cholestasis, variations in the enterohepatic circulation of bile acids and the gut microbiota (GM) characteristics differ between those with biliary atresia (BA) and non-BA, prompting a differential analysis of their respective GM profiles.
Using 16S rDNA gene sequencing to analyse the variance in GM composition among three groups: infants with BA (BA group, n=26), non-BA cholestasis (IC group, n=37), and healthy infants (control group, n=50). Additionally, correlation analysis was conducted between GM and liver function-related indicators.
Principal component analysis using Bray-Curtis distance measurement revealed a significant distinction between microbial samples in the IC group compared to the two other groups. IC-accumulated co-abundance groups exhibited positive correlations with aspartate aminotransferase, alanine aminotransferase, total bilirubin, direct bilirubin, and total bile acid serum levels. These correlations were notably reinforced upon the exclusion of microbial samples from children with BA.
The varying "enterohepatic circulation" status of bile acids in children with BA and non-BA cholestasis contributes to distinct GM structures and functions. This divergence underscores the potential for targeted GM interventions tailored to the specific aetiologies of cholestasis.
在患有胆汁淤积症的婴儿中，胆汁酸的肠肝循环和肠道微生物群（GM）特征在胆道闭锁（BA）和非 BA 之间存在差异，这促使我们对它们各自的 GM 图谱进行差异分析。
使用 16S rDNA 基因测序分析三组婴儿的 GM 组成差异：胆道闭锁（BA 组，n=26）、非 BA 胆汁淤积（IC 组，n=37）和健康婴儿（对照组，n=50）。此外，还对 GM 与肝功能相关指标进行了相关性分析。
基于 Bray-Curtis 距离测量的主成分分析显示，IC 组的微生物样本与其他两组有显著差异。IC 累积共现组与天冬氨酸转氨酶、丙氨酸转氨酶、总胆红素、直接胆红素和总胆汁酸血清水平呈正相关。在排除 BA 患儿的微生物样本后，这些相关性得到了显著增强。
BA 和非 BA 胆汁淤积症患儿胆汁酸的“肠肝循环”状态的差异导致了不同的 GM 结构和功能。这种差异突显了针对胆汁淤积症特定病因的靶向 GM 干预的潜力。
