Silva-Neto Habdias A, Barbeira Paulo J S, Coltro Wendell K T, Piccin Evandro
Departamento de Química, Universidade Federal de Minas Gerais, MG 31270-400, Brazil; Instituto de Química, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil.
Departamento de Química, Universidade Federal de Minas Gerais, MG 31270-400, Brazil.
Food Chem. 2024 Jun 30;444:138677. doi: 10.1016/j.foodchem.2024.138677. Epub 2024 Feb 15.
In this study, compact 3D-printed carbon black (CB) electrodes were manufactured for using in folic acid (FA) analysis in fruit samples. Before application in FA analysis, the electrode surfaces were characterized by high-resolution scanning electron microscopy and voltammetry using well-known redox probes. Square wave voltammetric study presented linear responses in the range between 10 and 200 µmol/L (R > 0.99), exhibited a suitable detection limit (LOD) of ∼ 5.1 µmol/L and acceptable performance in terms of reproducibility and anti-interference experiments. The analysis of FA in four different food samples using the proposed method agreed statistically with a comparative technique based on spectrophotometric measurements. Moreover, results from photostability experiments indicated that FA can be degraded after 5 and 20 min of UV exposure. These results successfully demonstrated the analytical feasibility of the 3D-printed electrodes as sensing material and for monitoring the photostability of FA in different fruit matrices.
在本研究中，制造了紧凑型3D打印炭黑（CB）电极，用于水果样品中叶酸（FA）的分析。在应用于FA分析之前，使用著名的氧化还原探针通过高分辨率扫描电子显微镜和伏安法对电极表面进行了表征。方波伏安研究表明，在10至200 µmol/L范围内呈线性响应（R > 0.99），检测限（LOD）约为5.1 µmol/L，在重现性和抗干扰实验方面表现出可接受的性能。使用所提出的方法对四种不同食品样品中的FA进行分析，在统计学上与基于分光光度测量的比较技术一致。此外，光稳定性实验结果表明，FA在紫外线照射5分钟和20分钟后会降解。这些结果成功证明了3D打印电极作为传感材料以及监测不同水果基质中FA光稳定性的分析可行性。
