Lemp M A, Dilly P N, Boyde A
Cornea. 1985;4(4):205-9.
We have utilized a radically new type of optical scanning microscope to study the full-thickness morphology of the intact cornea in an excised human eye bank eye and in freshly sacrificed rabbit eyes in situ. This technology enables one to study corneal morphology layer by layer in extremely thin sections, only disturbing the tissue with an applanating tip. We have demonstrated the cells of the corneal surface, subsurface cells, the topography of Bowman's membrane, corneal lamellae, stromal keratocytes, and the corneal endothelium. The application of this technology lends itself to the in vivo examination of the human cornea. This should aid us greatly in the study of normal morphology, disease states, and the reaction of the cornea in wound healing.
我们使用了一种全新类型的光学扫描显微镜，来研究取自人类眼库的离体眼球以及刚处死的原位兔眼中完整角膜的全层形态。这项技术能够让人在极薄的切片中逐层研究角膜形态，仅用一个压平尖端扰动组织。我们已经展示了角膜表面细胞、亚表面细胞、Bowman膜的形貌、角膜板层、基质角膜细胞以及角膜内皮。这项技术的应用有助于对人眼角膜进行活体检查。这将极大地帮助我们研究正常形态、疾病状态以及角膜在伤口愈合中的反应。
