Faculty of Pharmacy, Department of Microbiology and Immunology, Helwan University, POX 11795, Ain Helwan, Cairo, Egypt.
Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Zagazig University, Zagazig, Egypt.
BMC Res Notes. 2024 Feb 15;17(1):49. doi: 10.1186/s13104-024-06708-9.
Carbapenemase production and biofilm formation in K. pneumoniae are crucial factors influencing the pathogenicity and antibiotic resistance of this bacterium. This study investigated the interplay between carbapenemase production and biofilm formation in K. pneumoniae clinical isolates.
The distribution of biofilm-forming ability significantly differed between carbapenemase-producing (CP-Kp) (n = 52) isolates and carbapenemase-nonproducing (CN-Kp) isolates (n = 37), suggesting a potential link between carbapenemase production and biofilm formation. All the bla-harbouring isolates demonstrated biofilm formation, with varying levels classified as strong (33.33%), moderate (22.22%), or weak (44.45%). bla and bla-coharbouring isolates did not exhibit strong or moderate biofilm formation. bla and bla-coharbouring isolates were predominantly moderate (48.65%), followed by weak (32.43%), with none showing strong biofilm production. These findings suggest a correlation between the presence of carbapenemases and biofilm-forming ability; however, the heterogeneity in biofilm-forming abilities associated with different carbapenemase types and the absence of strong biofilm producers in the detected carbapenemase combinations prompt a closer look at the complex regulatory mechanisms governing biofilm formation in CP-Kp isolates.
肺炎克雷伯菌产生碳青霉烯酶和形成生物膜是影响该菌致病性和抗生素耐药性的关键因素。本研究探讨了肺炎克雷伯菌临床分离株中碳青霉烯酶产生与生物膜形成之间的相互作用。
产碳青霉烯酶（CP-Kp）（n=52）分离株和不产碳青霉烯酶（CN-Kp）（n=37）分离株的生物膜形成能力分布差异显著，提示碳青霉烯酶产生与生物膜形成之间可能存在关联。所有携带 bla 的分离株均表现出生物膜形成能力，根据强弱程度分为强（33.33%）、中（22.22%）或弱（44.45%）。携带 bla 和 bla 的分离株未表现出强或中生物膜形成能力。携带 bla 和 bla 的分离株主要为中（48.65%），其次为弱（32.43%），没有强生物膜产生。这些发现表明碳青霉烯酶的存在与生物膜形成能力之间存在相关性；然而，不同碳青霉烯酶类型相关的生物膜形成能力的异质性，以及检测到的碳青霉烯酶组合中没有强生物膜产生者，提示我们需要更仔细地研究 CP-Kp 分离株中生物膜形成的复杂调控机制。
