Department of Microbiology and Immunology, Kafrelsheikh University,Egypt.
Department of Microbiology and Immunology, Helwan University,Egypt.
J Pak Med Assoc. 2023 Apr;73(Suppl 4)(4):S274-S281. doi: 10.47391/JPMA.EGY-S4-54.
To genotypically assess the relationship between certain resistance and virulence determinants.
The cross-sectional study was conducted at Kafrelsheikh University, Egypt, from March 2019 to May 2021, and comprised pathologicalsamples, like blood,sputum, urine, vaginalswabs and wound swabs, that had been taken from patients who had never received treatment. The sample were collected from Kafrelsheikh and Mansoura University hospitals, and Klebsiella pneumoniae isolates were obtained. Resistance and virulence determinants were tested phenotypically. Uniplex polymerase chain reaction was used to evaluate the presence of several resistance accompanied genes and virulence genes in the isolates. Disc diffusion method was used to assess the isolates' susceptibility in accordance with the Clinical and Laboratory Standards Institute criteria for identifying diverse resistance patterns.
There were 23 isolatesfrom 16 patients. Of the tested isolates, 22(95.65%)showed drug resistance; 19(82.6%) had multidrug resistance, and 3(13.04%) had extensive drug resistance. There was no case of pan drug resistance. CTX-M-15, NDM, CTX-M-1, VIM-1 and qnr B genes were detected in 14(60.86%), 13(56.5%), 6(26.08%), 6(26.08%) and 6(26.08%) isolates, respectively. Moreover, 6(26.08%) isolates exhibited extended-spectrum β-lactamase producers, and 12(52.17%) ofsuch isolates contained both CTX-M-1 and CTX-M-15 genes, 6 and 33.3% contained CTX-M-1, CTX M-15 and fox genes. Type 3 fimbriae adhesin mrkD and mucoviscosity regulatory gene uge were found in the tested isolates. However, gene of iron uptake system kfu wasfound in 8(34.78%) isolates, and increased serum survival protein is and mucoviscosity accompanied gene magA were detected in 3(13.04%) isolates. A direct correlation was found among 5 from 8 Klebsiella pneumoniae virulence genes and antimicrobial resistance genes.
There was a direct correlation between the existence of virulence factors and resistance to antimicrobials.
从基因层面评估某些耐药性和毒力决定因素之间的关系。
本横断面研究于 2019 年 3 月至 2021 年 5 月在埃及卡夫拉谢赫大学进行，纳入从未接受过治疗的患者的病理样本，如血液、痰液、尿液、阴道拭子和伤口拭子。样本取自卡夫拉谢赫和曼苏拉大学医院，获得肺炎克雷伯菌分离株。对分离株进行表型耐药性和毒力检测。采用单重聚合酶链反应评估分离株中几种耐药伴随基因和毒力基因的存在情况。采用纸片扩散法根据临床和实验室标准协会标准评估分离株的药敏性，以确定不同的耐药模式。
从 16 名患者中分离出 23 株。在检测的分离株中，22 株（95.65%）表现出耐药性；19 株（82.6%）为多药耐药，3 株（13.04%）为广泛耐药。没有泛耐药株。14 株（60.86%）、13 株（56.5%）、6 株（26.08%）、6 株（26.08%）和 6 株（26.08%）分离株分别检测到 CTX-M-15、NDM、CTX-M-1、VIM-1 和 qnrB 基因。此外，6 株（26.08%）分离株为产超广谱β-内酰胺酶，其中 12 株（52.17%）含有 CTX-M-1 和 CTX-M-15 基因，6 株和 33.3%含有 CTX-M-1、CTX-M-15 和 fox 基因。在检测的分离株中发现了 3 型菌毛黏附因子 mrkD 和黏液调节基因 uge，但在 8 株分离株中发现了铁摄取系统 kfu 基因，在 3 株分离株中发现了血清生存蛋白 is 和黏液相关基因 magA。8 株肺炎克雷伯菌毒力基因与 5 株抗菌药物耐药基因之间存在直接相关性。
毒力因子的存在与对抗菌药物的耐药性之间存在直接相关性。
