Duan Renhe, Huang Jiahui, Zhang Donghan, Tian Enjing
Country Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, China.
Front Microbiol. 2024 Feb 1;15:1301085. doi: 10.3389/fmicb.2024.1301085. eCollection 2024.
section consists of lethal toxic mushroom species, causing many fatal poisoning incidents worldwide. Molecular techniques of nucleotide signatures and single nucleotide polymorphism (SNP) detection could be used to develop a specific method for identifying lethal section (sect.) species. A comparison of 38 sequenced and 228 validated sequences from sect. species showed a 17-base pair nucleotide signature and an SNP site between the lethal and non-lethal species. A specific minor groove binder probe was designed based on them. The results indicated that this method exhibited excellent specificity for the lethal subgroup, good detection in samples subjected to simulated gastric digestion (60 min boiling and 120 min digestion), and a 10 pg./μL detection limit. This method enables accurate detection of target species in samples under complex conditions and can provide evidence for poisoning incidents caused by lethal sect. species to assist in targeted treatment strategies.
该部分包含致死性有毒蘑菇物种，在全球范围内引发了许多致命中毒事件。核苷酸特征和单核苷酸多态性（SNP）检测的分子技术可用于开发一种鉴定致死性组物种的特定方法。对该组物种的38个测序序列和228个验证序列进行比较，结果显示致死性物种和非致死性物种之间存在一个17个碱基对的核苷酸特征和一个SNP位点。基于此设计了一种特异性小沟结合剂探针。结果表明，该方法对致死性子组具有优异的特异性，在模拟胃消化（60分钟煮沸和120分钟消化）的样品中检测效果良好，检测限为10 pg./μL。该方法能够在复杂条件下准确检测样品中的目标物种，并可为致死性组物种引起的中毒事件提供证据，以协助制定针对性的治疗策略。
