Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Molecules. 2022 Apr 6;27(7):2342. doi: 10.3390/molecules27072342.
plants generally contain ephedrine alkaloids, which are the critical precursor compounds of methamphetamine (METH). METH could cause serious physical and mental damage, and therefore materials are strictly in supervision internationally. However, unlawful utilization of herbs and its products still exist. Thus, it is imperative to establish a universal method for monitoring ingredients in complex mixtures and processed products. In this study, 224 ITS2 sequences representing 59 taxa within were collected, and a 23-bp genus-level nucleotide signature (GTCCGGTCCGCCTCGGCGGTGCG) was developed for the identification of the whole genus. The specific primers MH-1F/1R were designed, and 125 individuals of twelve species/varieties were gathered for applicability verification of the nucleotide signature. Additionally, seven batches of Chinese patent medicines containing herbs were used to test the application of the nucleotide signature in complex and highly processed materials. The results demonstrated that the 23-bp molecular marker was unique to and conserved within the genus. It can be successfully utilized for the detection of components in complex preparations and processed products with severe DNA degradation. The method developed in this study could undoubtedly serve as a strong support for the supervision of illegal circulation of -containing products.
植物通常含有麻黄碱生物碱，这是甲基苯丙胺（METH）的关键前体化合物。METH 会导致严重的身体和精神损伤，因此国际上对其材料进行严格监管。然而，非法利用草药及其产品的情况仍然存在。因此，建立一种通用的方法来监测复杂混合物和加工产品中的成分势在必行。在这项研究中，收集了代表 59 个种的 224 个 ITS2 序列，开发了一个 23 个碱基对的属水平核苷酸特征（GTCCGGTCCGCCTCGGCGGTGCG），用于鉴定整个属。设计了特异性引物 MH-1F/1R，并收集了 12 种/变种的 125 个个体进行核苷酸特征适用性验证。此外，还使用了七批含有草药的中药专利来测试核苷酸特征在复杂和高度加工材料中的应用。结果表明，23 个碱基对的分子标记是 特有的，在属内保守。它可以成功地用于检测复杂制剂和严重 DNA 降解的加工产品中的 成分。本研究中开发的方法无疑可以为监管含 产品的非法流通提供有力支持。
