Linfeng Lai, Xiaowei Zhou, Xueqin Chen, Xianfeng Zhu
Dingling Clinical College, Wenzhou Medical University, Wenzhou, China.
Wenzhou Central Hospital, Wenzhou, China.
Biomed Mater Eng. 2024;35(2):153-163. doi: 10.3233/BME-230068.
It is imperative to design a suitable material for bone regeneration that emulates the microstructure and compositional framework of natural bone while mitigating the shortcomings of current repair materials.
The aim of the study is to synthesize a 3D aerogel scaffold composed of PLCL/gelatin electro-spun nanofiber loaded with Simvastatin and investigate its biocompatibility as well as its performance in cell proliferation and ossification differentiation.
PLCL/gelatin nanofibers were fabricated in coaxial electrospinning with simvastatin added. Fibers were fragmented, pipetted into molds, frozen, and dried. The morphology of fibers and contact angles in 4 groups of PLCL, PLCL@S, 3D-PLCL, and 3D-PLCL@S was observed and compared. MC3T3-E1 cells were planted at the four materials to observe cell growth status, and ALP and ARS tests were conducted to compare the ossification of cells.
TEM scanning showed the coaxial fiber of the inner PLCL and outer gelatin. The mean diameter of the PLCL/gelatin fibers is 561 ± 95 nm and 631 ± 103 nm after the drug loading. SEM showed the fibers in the 3D-PLCL@S group were more curled and loose with more space interlaced. The contact angle in this group was 27.1°, the smallest one. Drug release test demonstrated that simvastatin concentration in the 3D-PLCL@S could remain at a relatively high level compared to the control group. The cell proliferation test showed that MC3T3-EI cells could embed into the scaffold deeply and exhibit higher viability in the 3D-PLCL@S group than other groups. The ossification tests of ALP and ARS also inferred that the 3D-PLCL@S scaffold could offer a better osteogenic differentiation matrix.
The PLCL/gelatin aerogel scaffold, when loaded with Simvastatin, demonstrates a more pronounced potential in enhancing osteoblast proliferation and osteogenic differentiation. We hypothesize that this scaffold could serve as a promising material for addressing bone defects.
设计一种适合骨再生的材料至关重要，该材料应模仿天然骨的微观结构和组成框架，同时减轻当前修复材料的缺点。
本研究旨在合成一种由负载辛伐他汀的聚乳酸-羟基乙酸共聚物/明胶电纺纳米纤维组成的三维气凝胶支架，并研究其生物相容性以及在细胞增殖和骨化分化方面的性能。
通过同轴电纺制备添加了辛伐他汀的聚乳酸-羟基乙酸共聚物/明胶纳米纤维。将纤维破碎，移液至模具中，冷冻并干燥。观察并比较聚乳酸-羟基乙酸共聚物、聚乳酸-羟基乙酸共聚物@辛伐他汀、三维聚乳酸-羟基乙酸共聚物和三维聚乳酸-羟基乙酸共聚物@辛伐他汀4组纤维的形态和接触角。将MC3T3-E1细胞接种于这四种材料上，观察细胞生长状态，并进行碱性磷酸酶（ALP）和茜素红染色（ARS）试验以比较细胞的骨化情况。
透射电子显微镜扫描显示内部聚乳酸-羟基乙酸共聚物和外部明胶的同轴纤维。负载药物后聚乳酸-羟基乙酸共聚物/明胶纤维的平均直径分别为561±95纳米和631±103纳米。扫描电子显微镜显示三维聚乳酸-羟基乙酸共聚物@辛伐他汀组的纤维更卷曲、疏松，交织空间更多。该组的接触角为27.1°，是最小的。药物释放试验表明，与对照组相比，三维聚乳酸-羟基乙酸共聚物@辛伐他汀中辛伐他汀的浓度可保持在相对较高水平。细胞增殖试验表明，MC3T3-E1细胞可深入嵌入支架，三维聚乳酸-羟基乙酸共聚物@辛伐他汀组的细胞活力高于其他组。ALP和ARS的骨化试验也推断三维聚乳酸-羟基乙酸共聚物@辛伐他汀支架可提供更好的成骨分化基质。
负载辛伐他汀的聚乳酸-羟基乙酸共聚物/明胶气凝胶支架在增强成骨细胞增殖和成骨分化方面具有更显著的潜力。我们推测这种支架可作为一种有前途的材料用于解决骨缺损问题。
