School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, 300070, Tianjin, China.
LangFang Health Vocational College, South Siguang Road, 065001, Langfang, China.
J Mater Sci Mater Med. 2019 Apr 12;30(4):47. doi: 10.1007/s10856-019-6249-3.
The objective of this study was to fabricate and characterize chitosan combined with different amounts of simvastatin-loaded nanoparticles and to investigate their potential for guided bone regeneration in vitro and in vivo. Different SIM-CSN formulations were combined into a chitosan scaffold (SIM-CSNs-S), and the morphology, simvastatin release profile, and effect on cell proliferation and differentiation were investigated. For in vivo experiments, ectopic osteogenesis and the critical-size cranial defect model in SD rats were chosen to evaluate bone regeneration potential. All three SIM-CSNs-S formulations had a porous structure and exhibited sustained simvastatin release. CSNs-S showed excellent degradation and biocompatibility characteristics. The 4 mg SIM-CSNs-S formulation stimulated higher BMSC ALP activity levels, demonstrated significantly earlier collagen enhancement, and led to faster bone regeneration than the other formulations. SIM-CSNs-S should have a significant effect on bone regeneration.
本研究的目的是制备和表征壳聚糖与不同载量辛伐他汀纳米粒的复合物，并研究其在体外和体内引导骨再生的潜力。不同的 SIM-CSN 配方被组合到壳聚糖支架中（SIM-CSNs-S），并对其形态、辛伐他汀释放曲线以及对细胞增殖和分化的影响进行了研究。对于体内实验，选择异位成骨和 SD 大鼠的临界尺寸颅骨缺损模型来评估骨再生潜力。所有三种 SIM-CSNs-S 配方均具有多孔结构，并表现出持续的辛伐他汀释放。CSNs-S 表现出优异的降解和生物相容性特征。4mg SIM-CSNs-S 配方刺激更高的 BMSC ALP 活性水平，显示出更早的胶原增强，并且比其他配方更快地促进骨再生。SIM-CSNs-S 应该对骨再生有显著影响。
